Study On The Gene Expressed Profile Of Di(2-ethylhexyl) Phthalate-induced Brain Tissue In Drosophila Melanogaster

Objective: To obtain the gene expressed profile of di(2-ethylhexyl)phthalate-induced brain tissue in Drosophila melanogaster, which will provideuseful clues to elucidating the toxicological mechanism of DEHP on thelifespan of Drosophila melanogaster at molecular level. Methods: the effectof DEHP on the lifespan of Drosophila melanogaster is learned,.and the bestconcentration is got for the gene expressed profile of di(2-ethylhexyl)phthalate-induced brain tissue by the technology of suppression subtractivehybridization and gene chip, then analyzed the data and JHEH1,MST57Da,ACP95EF, OS-E genes mRNAs further confirmed by real timeFQ-PCR. Results: the genes fragments were shown in differential expressedprofile with upregulated genes, including CYP6A2, CYP6A8, CYP6W1,CYP4P1, UGT36Bb, GST, Carboxylesterase, Glycine N-methyltransferase,Oxidoreductase, EST10, JHEH1, Oda, INAF, Grk, CG6908, CG1942,CG10560, CG11529, CG10553, CG1283, CG12505 and downregulatedgenes, including MST57Da, MST57Db, ACP95EF, OS-E, OS-C, PBPRP3,PBPRP1, A10, Arr2, RdgA, CG18628, CGl1391, CG18284, CG8424,CG5290, CG13947, CG15263.These genes involved in the functions ofsignal transduction, cell metabolism, growth, transcription factor andreproductive function. Conclusion:â‘ the magnitude of the toxicity relied onthe balance between phaseâ… reaction and phaseâ…¡reaction.â‘¡It wasspeculated that the slow-responsiveness of Drosophila melanogaster may berelated to DEHP disturbing nerve system coding activity.â‘¢DEHP may behazardous to the reproductive organs, visual system and growth of maleDrosophila melanogaster.