| The development of the heart involves a series of cellular migrations, fusions, and specific differentiations. During the past few years, remarkable progress has been made in identifying molecular signaling cascade involved in the heart development. The signaling pathways and transcription factors involved in heart development in DNA level have been studied in great details.The chick embryo has been intensively used as the material to study the mechanisms of mammal embryo development. However, specimens of similar ages post-fertilization may show a wide variation of maturation stages. The incubation times provide only an estimation of how far development of the embryo will have progressed. The temperature of incubation (which may be different among eggs depending on their locations within the incubator), as well as the rate of growth of the individual embryo itself will affect the overall developmental process. In this experiment, we established a series of standards to quickly estemited the actual stages of chick embryos, which can help us to identify the developmental age of chick embryonic heart through the outer morphologic criterion. Moreover, a silver stained 2D-gel of chick embryonic hearts at two stages of development (HH stage 19 and 24) was obtained, through isoelectric focusing on immobilized pH gradients followed by SDS-PAGE. The protein spots were detected and quantificated by the matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS) and EMBL (or SWISS-PROT) database searching.
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