| Ticks are well known vectors transmitting a great variety of infectious pathogens and causing serious damage to human and livestock. In ticks, an important biological feature is that they can reproduce many descendants, and male ticks have important function in this process. Thus, researches on spermatogenesis have important significance on the theoretical and practical level.A series of researches were conducted on the reproduction of male Haemaphysalis longicornis Neumann, including the structure and protein components of male reproductive system, changes of the protein contents and components during different developmental stages, the effects of juvenile hormone and molting hormone, using scanning electron microscope (SEM), Bradford method, SDS-PAGE, topical application and an in vitro culture methods. The present results will provide foundation for further investigations on reproductive biology of ticks. The main results were as follows:1. The male reproductive system of H. longicornis consisted of two tubular testes, paired vas deferens, ejaculatory duct and an accessory gland complex. The protein content of male reproductive system was complicate and protein diversity.2. The structure of testes changed obviously with the developmental stages. In unfed adults, the testes were small (Length: 960.00 m; Width: 163.48 m). On the day 2 after attachment, the testes increased obviously, the length and the width were 1573.70 m and330.44 m respectively. The testes reached its maximum at mating stage (Length: 2058.52 u m; Width : 490.10 m, occupied the most of tick coelum. After mating, the testes shrinked (Length: 1602.40 m; Width: 398.86 m).3. The protein level of the testes changed obviously during male developmental stages. In unfed males , the protein testes concentration was low (3.50 u g/tick), and increased rapidly on days 2 after attachment (15.00 u g/tick), which was 4.3 times than that of unfed male. Atthe mating stage, the protein level increased significantly and reached their maximum level (45.80 g/tick). After mating, the protein level content declined (26.58 g/tick).4. The protein content of the accessory gland complex had changed obviously with developmental stage, which was low in unfed stage (3.50 g/tick). Two days after attachment, it increased rapidly (8.68 g/tick), which was 2.5 times that of unfed male. On the mating stage, the level of protein content reached its peak (31.70 g/tick). After mating, the protein level decreased (22.88 g/tick).5. In unfed male, only 8 protein bands were detected in testes, on the day 2 after attachment, 8 new protein bands were found and 20 protein bands appeared at mating stage. After mating, the protein bands decreased to 15. In the four stages, the main bands was same, their molecular weights were 12.9KD, 24.9KD, 30.3KD, 43.3KD, 54.1KD, 58.3 KD, 69.4 KD and 88.5 KD respectively. On two days after feeding, the new appeared protein bands were 18.0 KD, 19.7KD, 26.3KD, 46.5KD, 50.0KD, 66.5KD, 74.5KD and 85.4KD. Comparing with day 2 after feeding stage, 15.9KD, 21.1 KD, 37.3 KD and 78.1 KD new protein bands were found at mating stage. From unfed stage to mating stage, the color of protein bands was deeper. After mating, five protein bands were disappeared, which molecular weights were 15.9KD, 21.1 KD, 37.3KD, 78.1 KD and 85.4 KD respectively.6. In unfed male, 7 main protein bands was detected in the accessory gland complex, there molecular weights were 26.3KD, 29.9KD, 30.6KD, 51.5KD, 57.0KD, 65.3KD and 85.3KD respectively. From the onset of feeding to mating stages, protein bands inceased obviously. Two days after feeding, 9 new appeared bands their molecular weights were 12.5KD, 13.9KD, 16.7KD, 18.4KD, 19.5KD, 20.7KD, 35.6KD, 51.8KD and 60.9KD. On mating stage, it added 5 bands, which molecular weights were 34.2KD, 42.1KD, 46.4KD, 68.5KD and 70.9KD. After mating, the protein added two bands than mating stage, which were 74.1KD and 77.1KD. However, the 13.9KD, 16.7KD, 18.4KD, 26.3KD and 34.2KD protein bands were reduced.7. Juvenile h...
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