| A series of systematic researches on the juvenile hormone (JH) structure, juvenile hormone esterase (JHE) activity and its change, female synganglion structure, and the location of neurosecretory cells which could promote the JH secretion in Haemaphysalis longicornis were investigated, by modern biological methods and related crossover techniques including Gas chromatography (GC), Mass spectrometry (MS), Radiochemical assay (RCA), Immunocytochemistry (ICC), biochemistry, and Scanning electron microscope (SEM) respectively. The main results were as follows.1. Using mass extraction method and GC, GC-MS, a component from whole body extracts of H. longicornis, which was similar to the insect standard JHIII in retention time and characteristic ion peak, was detected. The result revealed that there existed JHIII in H. longicornis. This was the first report in ticks.2. Juvenile hormone esterase (JHE), which could degenerate JH, was detected in female H. longicornis,. JHE changed with female developmental stages. The metabolite radioactivity of unfed, mating, engorgement, the first d3 and d5 after engorgement were 177.67dpm, 1030.67dpm, 2132.40dpm, 1448.80dpm, 1902.17dpm and 2134.67dpm respectively. This meant JHE activity had a peak on the day of engorgement.3. The synganglion of female H. longicornis was a high-connected and triangular structure. Its former was narrow and the back end was wide. SEM results suggested that the size of synganglion in unfed female was the smallest (L: 270.00um, W: 232.50um), and reached the largest size (L: 453.25um, W: 433.25um) until the engorgement stage along with the feeding. After engorgement, its size remained unchangeable.4. The ICC method was used to detect whether neurosecretory cell receptors, which could bind anti-Mas-AT, existed in female synganglion. The result revealed that no receptor was found in this organ, which could be the difference between the insect Mas-AT and the H. longicornis AT.
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