Preliminary Research On Tissue Culture Of C.Sinensis

Camellia is one of three main drinks in the world, it also has great edible and medical value. The paper reported something about it. The callus was induced and proliferated using young leave as explant, which comes out at early spring. In this paper, the author discussed the induction effect and the proliferation effect by using different kinds of hormone and different physical-chemical factors. The growth circle and some physiology-biochemical indexes were illustrated. As the same time, the tendencies of flavonids, saponins and tea-polyphenols during growth circle are analysed. The paper provides scientific and experimental basis for cell culture and secondary metabolism matters yield.The results are following:1.The effects of induction and the proliferation which mediums are applied different kinds of hormone or different concentration hormone are different.Medium ER is optimum for callus inducing and proliferation, but medium MS B5, N6 also can be used to induce callus. In callus inducing, 2,4-D2.0mg/L is the best of all single hormone, NAA1.0mg/L+6-BA1.0mg/L is the best of all combined hormone. In callus proliferation, NAA1.0mg/L+6-BA1.0mg/L is the best.The effects of different physical-chemical factors are different. The callus cultured in white light grows better than that in dark. When the concentration of glucose is 40mg/L, the effect of callus proliferation is the best of all.Activated carbon has no use in preventing callus browning, but protein tyrosine hydrolysate 0.5%does good.2.The change of physiology-biochemical norm:The growth circle of callus is 30 days and the growth-curve looks like "S". The growth circle could be divided three parts: Delay phase(0-12d), Index phase(12-24d)and Steady phase (24-30d).During the growth circle, the tendency soluble protein contents, SOD, POD activities were different during the three parts. They show some physiology -biochemical indexes character during proliferation and cell activities. It is different between growth-circle of callus cultured brightly and that of callus cultured darkly.3.The change of secondary metabolism matters content:During growth circle, flavonids, saponins and tea-polyphenols were connectedwith callus growing condition. Secondary metabolism matters synthetic and accumulated are also connect with growing condition. Secondary metabolism matters accumulated mainly during steady phage (24-30d). And when the callus cultured brightly, the three secondary metabolism matters content are higher than that of callus cultured darkly.