| In this paper, the experimental materials were primarily cultured nerve cells. By the methods of MTT colorimetric assay, measurement of enzyme activity, the phase contrast microscope, and combined with HPLC, we investigated that the influence of the pulsed electric field(f= 50HZ, Epp=lV/cm)on nerve cell survival ,antioxidant ability ,morphology and secretion of neurotransmitter .By MTT colorimetric assay, we investigated the influence of ELFEMF on primarily cultured nerve cells. The results revealed that survival of cell treated by the pulsed electric field could be improved in short exposure time, the rate of increase of which could reach to 12.3%. Difference was significant when p<0.01. when cell were exposed directly to the pulsed electric field. On the contrary the cell survival could be inhibited in longer exposure time. In the indirect effect and co-effect of culture medium, the inhibition on cell survival was significant, the rate of which reached to 10 to20%(p<0.001).The results revealed that pulsed electric field had promotive and inhibitory effects on cell survival, which was associated with time. And there were indirect and direct effect of the pulsed electric field on cell survival, and the two effects existed simultaneouslyAdopting the methods of reagent case and biochemical inspection, we study the influence of the pulsed electric field on the content of MDA, SOD activity, and the content of NO. The results indicated that the nerve cell antioxidant ability could be greatly improved in short exposure time (t=5minutes), which represented the increase of SOD activity and the content of N0,but the decrease of the content of MDA. Difference were considered significant when p<0.05. On the contrary the antioxidantability could be greatly decreased in long exposure time, which represented the apparent inhibition of SOD Activity, the rate of inhibition of which reach tol8.83%.(p<0.001), at the same time the significant decrease of the content of NO, the rate of decrease of which reached to 19.25%. Accordingly, the degree of lipid peroxidation in cell body was increased, and the content of MDA was increased to 2.038±0.24m mol/mg protein, the rate of variety of which was 12.04%. From above we could infer the ELFEMF had a direct effect on the cell by the influence on free radical metabolism.The experimental material were primarily cultured nerve cells By the phase contrast microscope, we investigated the influence of ELFEMF on nerve cell morphology. The results indicated that the cell growth could be accelerated in short exposure time, which represented the round cell body, excellent refraction, remarkable colonies, plenty of synapsis relation ship among these colonies, and a small number of isolated cells. When the cells were exposed to the pulsed electric field for 10 minutes, there was no significant difference between normal cell and treated cell. The growth of cell, however, could be significantly inhibited. When the cell were treated for 15 to 30 minutes, Which represented the unremarkable colonies, bad refraction, fracture and shrinking of synapsis, and a lot of isolated cells among these colonies.The result declared that the PEF influenced the development and differentiation of nerve cell, which was characterized by "window", that is "frequency", "power window" and "time window"(which displayed the selectivity to frequency, power and time).Adopting the primarily cultured nerve cell of SD-mouse embryo, by the effect of ELFEMF: (1)The co-effect of nerve cell and culture medium.(2)The indirect effect of culture medium.(3)The direct effect of nerve cell, and combined with HPLC, We measured the concentration of four kinds bioamines in the culture medium. The results showed the concentration of E and DA was increased by short time stimulation of the PEF. In the co-effect of nerve cell and culture medium, the concentration of E is increased to 4.56 x 10-6g/L,the rate of variety of which reached to19.6%(p<0.001).Also the content of the metabolin of DA and 5-HT, 5-HIAA and HVA, is significant in...
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