Application Of Genetic Markers In Caragana Fabr

Biological characters were observed and karyotypes were analyzed of Caragana microphylla Lam C.intermedia Kuang et H.C.Fu and C.arborescens(Amm.)Lam in this paper. At the same time ,thirty plant belong to Caragana Fabr from different regions were analyzed by molecular markers in order to study relationship among them. The main results as followed:1 .The result of morphology showed: there are differentia among Caragana microphylla Lam C.intermedia Kuang et H.C.Fu and C.arborescens(Amm.)Lam. Such as stem with hairness or smooth ,highbrush or underbrush, stronger resistance or weaker resistance. There are similitude among them such as stem with litter leaf or pinprick from stipule.2.The result of cytology showed: The chromosome number of Caragana microphylla Lam is 2n=16. The karyotype of Caragana microphylla Lam can be espressed as 2n=16=16m(4SAT); The chromosome number of C.intermedia Kuang et H.C.Fu is 2n=18. The karyotype of C.intermedia Kuang et H.C.Fu can be espressed as 2n=18=16m(4SAT)+2M; The chromosome number of C.arborescens(Amm.)Lam is 2n=20. The karyotype of C.arborescens(Amm.)Lam can be espressed as 2n=20=20m(4SAT); From the conclusion one and two ,we can guess: the C.intermedia Kuang et H.C.Fu'may be the hybrid or intermediary genve between Caragana microphlla Lam and C.arborescens(Amm)Lam ,also may be have different haploid chromosome number.3.The result of RAPD showed: Caragana Fabr from different regions have far or near relationship, they may devide into four parts. The first population is Caragana microphylla Lam. The second population is C.spinosa(L.)DC. The third population is C.ching haiensis Lion filia.sp. The fourth population is C.purdomii Rehd.