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Studies On Microstructure & Chemical Components Of Organs And Embryonic Development And Fixing Nitrogen In Elaeagnus Conferta

Posted on:2004-08-13Degree:MasterType:Thesis
Country:ChinaCandidate:Y M LiuFull Text:PDF
GTID:2120360092985849Subject:Botany
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The microstructure and chemical components of organs, embryonic development and nitrogen fixation in Elaeagnus conferta in this paper were investigated. The results showed:1. The microstructure of root, stem, leaf and pollenSimilar character on the structure of root, stem and leaf was that they all had only one layer cuticle, thin epidermis, developed xylem, lots of closely-arraged vessels, and these showed the wet-resistive and hot-resistive features. The tricolporate pollen was nearly globe-shape, 27.15(25-32)μ m ×27.35(25-31)μ m, The colpus was short which edge was not smooth. The interal pore which was big and circle-shape clearly protruded.The pollen's exine had outer and interal layers and the ornamentation was smooth.The outer layer was 0.4 u m, clearly thicker than the internal one. All of these could be used as the scientific basis for pharmacentical identification of Elaeagnus conferta Roxb.2. The structure and function in 3 stages of development of root nodules in Elaeagnus confer/aFrankia existed among the cells of nodule cortex before infecting them. The infected cells were restricted to the middle region of the nodule cortex. At early stage of invasion, the filamentous hyphae were branched and septate. Lipid bodies were distributed in the young infected cell. In mature nodules, the number of infected cells was much larger than that in both young and senescent nodules. The terminal of endophyte hypha swelled to form lots of vesicles which were septate and double-wall. Vesicles played an importatant role in nitrogen fixation. In senescent nodules, the vesicles were non-septate and disintegrated. The nitrogen fixation and hydrogen uptake activities in mature nodule were much higher than those in both young nodules and senescent nodules.3. Sporogenesis and gametogenesisThe microsporein's rate of development was higher than that of macrospore. The microsporein finished meiosis I while the macrospore was still archesporial cell. The macrospore's development quickened after fertilization and finished in short time, nearly within Id. The mature pollen grains were 3-celled and the tapetum belonged to the secretory type. The character of macrospore in development were as followed: anatropal ovule, basal placenta, thick nucellar, two integument, megaspore mother cell coming directly from archesporial cell, early degenerating antipodal cell, thedevelopment of the embryo sac being conformed as the polygonum type.4. Primary identification of fruit pigmentThe carotenoids were separated into seven chromatogram by TLC on fibre and silica gel with an eluant of petroleumether/ acetone/ benzene (2:0.5:2V/V). The maximum carotenoid was identified as y-carotene by UV-visible and IR spectrum.5. Analysis on the components of seed oilThe content of seed oil was 1.98%. It contained 6 kinds of fatty acid (FA) including oleic acid (C18:1) 34.15%, linoleic acid (C18:1) 31.51% and linolic (C18: 3)14.50%. It had only two kinds of saturated fatty acid (SEA) which were palmitc acid (C16:0)13.83% and stearic acid (C18:0) 2.88%. Unsaturated fatty acid (UFA) was dominant in seed oil, especially oleic acid and linoleic acid. The content of UFA was over 83%. The proportion of UFA to SFA was 4.98 while SFA : MUFA : PUFA was 1 : 2.23 : 2.75. Seed contained much beneficial mineral elements such as K 0.784%, Fe30.99 mg/kg, Zn 10.13 mg/kg etc.6. Chemical components of organsThe properties of chemical components were showed as followed: tannin 8.11% (matural leaf), 7.70% (young leaf), 4.16% (bark); flavones 0.83% (matural leaf), 0.51% (young leaf), 0.45% (bark), vitaminC HOmg/g (matural leaf), 116mg/g (young leaf), lOmg/g (bark); crude protein 14.31% (matural leaf) ,18.25% (young leaf) ,3.19% (bark); total sugar 22.50% (matural leaf), 25.75% (young leaf), 26.00% (bark); pectin 2.59% (matural leaf> 3.92% (young leaf), 0.92% (bark); fibre 30.29% (matural leaf), 29.24% (young \eaf), 62.60% (bark). It also contained vatious essential amino acids. The result showed that the leaves (including matural and...
Keywords/Search Tags:Elaeagnus conferta Roxb, ultrastructure, nitrogen fixation, embryonic development, chemical components, development and utilization
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