Studies On The Tissue Culture And The Metabolic Enzymes About Salidroside In Tibet Rhodiola Rosea

The Tibetan folk medical plant Rhodiola rosea was usually planted in a special environment in alpine plateau areas, such roots of which are used to cure senility,fatigue in man, and also to improve human immunity so as to be recovered from many diseases significantly. In this paper,we studied MS medium was used for the callus and bud, and MS or B5 medium were used for the root induction of both the leave and the stem of the Rhodiola rosea. MS and B5 medium supplemented with different ration of hormone and concentration sucrose and agrose. The Salidroside is the one of the most pharmacological activity-qualitity matters in Rhodiola rosea, and the determination of which in the callus of Rhodiola Kirilowir was made by HPLC .Then we studied the activity of enzymes PAL ,CA4H and TAL which may be the important enzymes in the metabolic pathway of Salidroside in the callus of Rhodiola Kirilowir. The results showed that:(l)The best mediums to the callus of the Rhodiola rosea were MS+6BA 2.0mg L-1+NAA0.2mg L-1,MS+6BA 3.0mg L-1+NAA0.3mg L-1,MS+6BA 2.0mg L-1 +IAA0.25mg L-1. And it was easier to the induction of the callus for leave than stem ,the IAA and GA was extremely remarkable to the bud growth.The best medium to the root induction were MS and B5+30g L-1 sucrose+8g L-1agrose+IAA 0.5mg L -1 with the light of 8 h d-1 and pH5.8,the difference between the two mediums was that the root of the former was thinner and longer than the latter.(2)The explant ,the temperature and the growth era of the callus for Rhodiola Kirilowir all worked on both the content of the Salidroside and the activity of enzymes including PAL ,CA4H and TAL in the callus. With 24 C or 4 C, the change trends of the content of the Salidroside was basically consentaneous in the consecutive cultural eras of the callus. In the callus from the different explants,the influence was maximal to the the activity of PAL enzyme and the influence was lowest to the the activity of CA4H enzyme, and the influence to the the activity of TAL enzyme was ascertained according to the explant of the callus. So in the same explant with the different temperature or in the different explant with the same temperature, there was no incident between the content of the Salidroside and the activity of enzymes PAL ,CA4H and TAL, and We presumed that there may be emphasized particularly on different metabolic pathway of Salidroside. According to the conserved domain of the PAL gene family, we achieved to the segment of PAL gene about 950bp in Rhodiola crenulata .Some proposals were put forward for further development of the important enzymes in the metabolic pathway of Salidroside.