Studies Of Several Biochemistry Indexes In Chinese Mitten-handed Crab And Chinese Shrimp

Crustacea have not specific immunity, phagocytosis of haemolymph is the main defense mode to exogenous matter. In the process of phagocytosis , lots of activity matter produce and set free , the most major is oxygen free in them. This dissertation studies superoxide dismutase(SOD), catalase(CAT), malondiadehyde(MDA), lysozyme(LSZ), acid phosphatase(ACP), alkaline phosphatase(ALP), phenoloxidase(PO) etc 7 kinds of activity matter by NET reduction method, ammonium molybdate, TBA method, flat method, bisodium benzene phosphate, L-dopa etc 6 kinds of method. The following is results:Under situation of hypoxia or phagocyte activated, organism produce lots of free radicals, 02~ is the most important. When it surpasses the removal ability of antioxidation , it .causes chain reactions of peroxidation, resulting in serious damnification of organism membrance and microcycle, even collapse. Free radicals and product of their metabolism make polyamylose divide chains, unsaturated fat acid peroxide, protein tangle, RNA and DNA rupture and mutate. SOD can remove O2 specifically.The activity of SOD of juvenile Chinese mitten-handed crab of disembarkation syndrome is respectively 8.6206,7.0608 and 6.4524 U/mg protein; that in the control juvenile Chinese mitten-handed crab is respectively 35.0746,36.0926 and 35.4975 U/mg protein. SOD in the disease crabs is remarkably lower than that in the control crabs(P<0. 01).The activity of SOD of Chinese shrimp of white spot syndrome is respectively -0.2281, -0.3632 and -0.3310 U/mg protein; that in the control Chinese shrimp is respectively 2.2449,2.2471 and 2.2460 U/mg protein. SOD in the disease shrimps is remarkably lower than that in the control shrimps(P<0. 01).The activity of SOD of adult Chinese mitten-handed crab of pectoral appendages trembling disease is respectively 9.3714,9.8065 and 10.0007 U/mg protein-, that in the control adult Chinese mitten-handed crab is respectively 1. 6488, 1. 6562 and 1. 6653 U/mg protein. SOD in the disease crabs is remarkably higher than that in the control crabs(P<0. 01).The activity of CAT of juvenile Chinese mitten-handed crab of disembarkation syndrome is respectively 10. 7855, 12. 8221 and 11. 8378 KL'/mg protein-, that in the control juvenile Chinese mitten-handed crab is respectively 29. 1108,27.4594 and 28. 1518 KU/mg protein. CAT in the disease crabs is remarkably lower than that in the control crabs(P<0. 01).The activity of CAT of Chinese shrimp of white spot syndrome is respectively 8. 0269, 7. 5373 and 7. 5373 KU/mg protein; that in the control Chinese shrimp is respectively 12. 8156, 11. 1229 and 11. 5331 KU/mg protein. CAT in the disease shrimps is remarkably lower than that in the control shrimps(P<0. 01).The activity of CAT of adult Chinese mitten-handed crab of pectoral appendages trembling disease is respectively 1.5004,0. 7317 and 0. 3419 KU/mg protein; that in the control adult Chinese mitten-handed crab is respectively 0. 7379,0. 7229 and 1. 5079 KU/mg protein. CAT in the disease crabs have not remarkably distinction with that in the control crabs(P>0. 05).The content of MDA of juvenile Chinese mitten-handed crab of disembarkation syndrome is respectively 0. 7690, 0. 7666 and 0. 8143 nL TEP/mg protein; that in the control juvenile Chinese mitten-handed crab is respectively 0.4567,0.4723 and 0.4334 nL TEP/mg protein. MDA in the disease crabs is remarkably higher than that in the control crabs(P<0.01).The content of CAT of Chinese shrimps of white spot syndrome is respectively 0.8460,0.8700 and 0.8500 nL TEP/mg protein; that in the control Chinese shrimp is respectively 0. 8680, 0. 8440 and 0. 8440 nL TEP/mg protein. MDA in the disease shrimps have not remarkably distinction with that in the control shrimps(P>0.05).The content of MDA of adult Chinese mitten-handed crab of pectoral appendages trembling disease is respectively 0. 7380,0.7468 and 0.7502 nL TEP/mg protein-, that in the control adult Chinese mitten-handed crab is respectively 0. 3873, 0. 3880 and 0. 3873 nL TEP/mg protein. MDA in the dise...