Function Analysis Of Nucleoside Diphosphate Kinase In Response To The Stress Of Phenanthrene

Polycyclic aromatic hydrocarbons (PAHs) are of global environmental concern because they can cause many health problems such as cancer, mutation and so on in human. Plants are very important in removing PAHs from the atmosphere and soil. But the mechanism of plants how to respond to PAHs stress is still not clear at presant.On the basis of differentially proteome analysis, we successfully constructed NDPK family's overexpression(OE) vectors, and transformed them into Wide type (WT) Arabidopsis thaliana (Col0 as the gene background). Observation NDPK3 OE and T-DNA insertion lose of function mutants (SALK₁38260C) exposed to different concentration of phenanthrene,we found that root hairs, chlorophyll content were reduced as the concentration increased. At the same time, 3,3'-diaminobenzidine (DAB) stain after exposed to different concentration of phenanthrene for 15 days, the brown getting stronger as the concentration increased too. Suggesting that the content of H2O2 also increased. In addition to, we also found the malonadehyde (MDA) content were promoted in WT, NDPK3 OE mutants and T-DNA insertion lose of function mutants compare with that of a non-treated control sample; MDA content were significantly increased in SALK₁38260C than that in WT and NDPK3 OE mutants under the stress of 0.1mM phenanthrene. That's to say, MDA plays an important role in reducing membrane lipid peroxidation. Besides, POD and CAT activity were promoted significantly in NDPK3 OE mutants under the stress of 0.1mM phenanthrene than WT and SALK₁38260C. It suggest that NDPK3 OE mutants enhanced tolerance to phenanthrene. Reveal that NDPK3 response to phenanthrene stress by regulating the oxidative stress.