| As the development of modern industry, especially the wide use of fossil fuels, the level of polycyclic aromatic hydrocarbons (PAHs) become higher and higher in environment. It is reported that PAHs can cause monstrosity, cancer and mutation, so it's harmful to organism. Therefore, the further studies on how animals and plants respond to PAHs stress will provide theoretical basis on prevention and management of PAHs. Plant can absorb PAHs from atmosphere and soil, then generate a certain stress response. However, the molecular mechanism of plant responded to PAHs stress is not clear yet. In this study, on the basis of the differential proteomics studies to phenathrene stress onto Arabidopsis thaliana, nucleotide sequences of related genes were BLAST-searched on the TAIR database (http://www.arabidopsis.org/). Then fuctional analysis of these proteins was conducted by the approach of gene activation and inactivation. The main results were as follows.Nine over expression vectors (At4g11010, At2g36460, At3g52960, At4g38740, At2g44060, At1g11860, At5g17920, At1g50480, At1g70890) were constructed, and transformed into Arabidopsis thaliana plants with Agrobacterium tumefaciens -mediated transformation method. The semi-quantitative PCR was use to screen and identify the activation plants of four genes (At4g11010, At2g36460, At3g52960, At4g38740). Results showed that one line of activation plant of At4g11010 was more sensitive to PAHs stress on root length, which is shorter than that of wild-type. In the meantime, the loss of function T-DNA insertion mutants of these genes were also used to study the changes of phenotype under the phenanthrene stress. Results showed that all mutants had no difference from the wide-type. In a word, our results may proviede a scientific basis for understanding signal transduction pathway regulating PAHs stress in plants, and suggest that the molecular mechanism of Arabidopsis thaliana in responding to PAHs stress is very complicated.
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