Comparison Of Mitochondrial Transplantation By Using A Stamp-type Multi-needle Injector And Platelet-rich Plasma Therapy For Hair Aging In Naturally Aging Mice

The mechanism of hair loss caused by aging is related to mitochondrial dysfunction.Pep-1-mediated mitochondrial transplantation is a potential therapeutic application for mitochondrial disorders,but its efficacy against hair aging remains unknown.This study compared platelet-rich plasma(PRP)therapy with mitochondrial transplantation for hair restoration and examined the related regulation in naturally aging mice.After dorsal hair removal,100 week old mice received weekly unilateral injections of 200 ?g of allogeneic mitochondria-labeled 5-bromo-2?-deoxyuridine with(P-Mito)or without Pep-1 conjugation(Mito)or human PRP with a stamp-type electric injector for 1 month.The contralateral sides were used as corresponding sham controls.Compared with the control and corresponding sham groups,all treatments stimulated hair regrowth,and the effectiveness of P-Mito was equal to that of PRP.However,histology revealed that only P-Mito maintained hair length until day 28 and yielded more anagen follicles with abundant dermal collagen equivalent to that of the PRP group.Mitochondrial transplantation increased the thickness of subcutaneous fat compared with the control and PRP groups,and only P-Mito consistently increased mitochondria in the subcutaneous muscle and mitochondrial DNA copies in the skin layer.Therefore,P-Mito had a higher penetrating capacity than Mito did.Moreover,P-Mito treatment was as effective as PRP treatment in comprehensively reducing the expression of aging-associated gene markers,such as Mitochondrial ribosomal protein S5(IGF1R)and Mitochondrial ribosomal protein S5(MRPS5)and increasing anti-aging Klotho gene expression.This study validated the efficacy of mitochondrial therapy in the restoration of aging-related hair loss and demonstrated the distinct effects of PRP treatment.