Structural Characteristics Of Gut Microbiota And Its Correlation With Metabolism And Inflammation In Non Obese Patients With Nonalcoholic Fatty Liver Disease

Objective: By comparing the clinical characteristics of the patients with different types of non-alcoholic fatty liver disease(NAFLD),the changes of microbiota and their metabolites,and the relationship between the two mentioned above,the aim is to clarify the difference and potential significance of gut micrbiota in non-obese and obese NAFLD patients.Methods: The study included 80 male subjects in four groups: 20 in group 1(non-obese NAFLD group),20 cases in groups 2(obesity NAFLD group),20 cases in groups 3(health check-up group)and group 4(normal metabolic obesity group)respectively.Enzyme-linked immunosorption assay(ELISA)was used todetect the metabolites and inflammatory factors CK-18,IL-17,LPS,SCFAs and TMAO in four groups subjects.High-volume sequencing was carried out using 16 S r DNA amplification subsequencing technology in the V4 region of the 16 S r DNA gene of the structural characteristics of the microbiota of the four groups of the study subjects were identified by bio-information analysis.Finally,the statistical software was used to analyze the correlation between different genus of non-obese NAFLD and obese NAFLD patients with metabolic and inflammatory factors.Results: 1)From the clinical characteristics,hyperlipidemia,hyperuricemia,BMI,waist circumference,hip circumference,systolic pressure,hemoglobin,uric acid,glutamate transaminase,total bililine,direct bililine,lactic acid dehydrogenase,triglycerides,total cholesterol,LDL differences between the four groups are statistically significant(P<0.05).BMI,hyperlipidemia,hyperuricemia,uric acid,and total cholesterol are independent risk factors affecting non-obese NAFLD(OR values 1.09,1.12,1.14,1.01,7.05),and triglycerides are non-prone factors affecting non-obese NAFLD.Differences in serum CK-18,LPS and SCFAs content were statistically significant between the four groups(P < 0.05);Based on the single-factor analysis,it is concluded that there are statistical differences in the distribution of CK-18,LPS and SCFAs serum inflammatory factors in four groups(P<0.05).By comparing the three factors with Bonferroni(Table 1-5),it was concluded that the CK-18 content of the obese NAFLD group was higher than that of the non-obese health check-up group(P=0.038)and the obese health check-up group(P=0.018;LPS in the obese NAFLD group was higher than in the obesity health check-up group(P=0.041);and SCFAs in the obese NAFLD group was higher than in the Obese Health Check-up Group(P=0.048).2)The community abundance of non-obese NAFLD group and obese NAFLD group was lower than that of healthy control group and metabolic normal obesity control group,and the community abundance of non-obese NAFLD group was higher than that of obese NAFLD group.α Non-obese NAFLD group and obese NAFLD group of 3 bacterial: cyanobacteria,deformed bacteria,thick γ-walled bacteria;Root tumor bacteria,γ-deformation bacteria,Lactobacillus;5 departments: cyanobacteria,root tumor bacteria,Nesseria,Bayerink bacteria,Lactobacillus;2 genus: tablets,Nesseria,there are significant differences between the department.The thick-walled bacteria abundance of the non-obese NAFLD group was lower than that of the obese NAFLD group.In the non-obese NAFLD group,the content of deformed bacillus was higher,and the content of the genus E.coli,E.coli and immobilized bacteria was higher than that of the other 3 groups in the non-obese NAFLD group.The meaningful species in the obese NAFLD group are thick-walled bacteria,banana spores,Verongoccus,and giant monocytobacteria.3)"Gate" level,non-obese NAFLD group cyanobacteria gate abundance and Cr,UA negative correlation coefficient of-0.7157,-0.4520;In the obese NAFLD group,thick-walled bacterial plumpness was positively related to TMAO,with a correlation coefficient of 0.4526,deformed bacterial abundance was positively related to LPS,the correlation coefficient was 0.6105,and the hip circumference was negatively related,and the correlation coefficient was-0.5070.At the "gang" level,the abundance of spores belonging to thick-walled bacteria in the non-obese NAFLD group was negatively related to hip circumference,with a correlation coefficient of-0.5775.The abundance of deformed bacteria in the obese NAFLD group was positively related to LPS,the correlation coefficient was 0.5654,the correlation coefficient was negative with hip circumference,and the correlation coefficient was-0.5439;At the "eye" level,the emulsion of Lactobacillus,which belongs to thick-walled bacteria in the non-obese NAFLD group,was positively related to waist and hip circumference,with a correlation coefficient of-0.450,-0.584;The abundance of deformed bacteria in the obese NAFLD group was negatively related to waist circumference,hip circumference and TBil,and the correlation coefficient was-0.6318,-0.5002,-0.4689.Conclusion: 1)BMI,hyperlipidemia,hyperuricemia,uric acid and total cholesterol are independent risk factors affecting non-obese NAFLD,and triglycerides are non-prone factors affecting non-obese NAFLD;2)There are abnormal expressions of serum inflammatory factors and lipid metabolism indicators in non-obese NAFLD patients,and there is a close relationship between inflammation and lipid metabolism and non-obese NAFLD,and the relationship between them deserves further study;3)The microbiotal structure of non-obese NAFLD patients was better than that of obese NAFLD patients;Bacillus deformation and Lactobacillus lactic acid were the dominant bacteria in the non-obese NAFLD group;4)Non-obese NAFLD patients significantly increased the sterilization of mid-cyanobacteria bacteria gate abundance with Cr,UA negative correlation,decreased thick-walled bacterial amphithyrosis and SCFAs,TBil,hip circumference negative correlation;5)It is speculated that the dominant bacteria of the gut microbiota in the non-obese NAFLD population may be one of the causes of the disease,but because the micro-ecology of the gut microbiota is too complex to be determined by just a few genus,it needs to be further studied.