Effect Of Apoptosis-promoting Mechanism Of TIPE1 Expression On Ovarian Cancer And Granulosa Cells

Background:Many studies have shown that TIPE1 is involved in cell apoptosis in various cancer tumors,which can predict the prognosis of patients,and is a kind of cancer recognition biomarker.However,in ovarian cancer,the clinical significance and biological function of tipe1 have not been elucidated.The apoptosis of ovarian granulosa cells can affect follicular development.Therefore,to study the role of TIPE1 in ovarian granulosa cells,granulosa cells are large cell groups around the egg,so as to explore the possible impact of tipel on the development environment of oocytes.Objectives:To explore the clinical significance of TIPE1 expression in ovarian cancer;the role of TIPE1 in ovarian cancer and granulosa cells,and the mechanism of TIPE1 in promoting apoptosis.Methods:1.Detect the expression of TIPE1 in ovarian cancer tissues with tissue chip immunohistochemistry,qRTPCR,western blotting and other methods,analyze the relationship between the expression of TIPE1 and the degree of malignancy in 29 clinical samples of ovarian cancer,and 90 clinical samples of ovarian cancer The relationship between the expression of TIPE1 and survival prognosis.2.Establish a stable TIPE1 high-expressing ovarian cancer cell line(A2780/SKOV-3)through infection with lentivirus,construct an ovarian cancer cell model,and observe the effects of TIPE1 on the ovaries using CCK-8,clone formation experiment,flow cytometric apoptosis experiment,Balb/c nude mice tumor formation experiment,etc.The effect of cancer cell viability and apoptosis.3.Establish a stable granular cell line with high TIPE1 expression through infection with lentivirus,construct an ovarian granular cell model,use CCK-8,clone formation experiment,flow cytometric apoptosis experiment,electrochemiluminescence method,etc.to observe the effect of TIPE1 on granular cells The effect of activity and apoptosis.4.Analyze the expression of caspase 3,caspase 8 and caspase 9 in ovarian cancer cells and granulosa cell models by using qRTPCR,western blotting,tissue immunofluorescence experiments and other methods,and explore the effects of TIPE1 in ovarian cancer cells and granulosa cells The influence of apoptotic protein caspases expression.In ovarian cancer cells and granulosa cells that highly expressed TIPE1,z-VAD was used to inhibit the expression of caspases,and it was verified that TIPE1 can promote apoptosis by promoting the expression of caspases.Results:1.TIPE1 is low expressed in ovarian cancer tissues and cell lines;There is a negative correlation between TIPE1 expression level and poor prognosis of patients with ovarian cancer.2.Both in vivo and in vitro experiments have confirmed that the high expression of TIPE1 can inhibit the activity and proliferation of ovarian cancer cells,and induce apoptosis.3.The high expression of TIPE1 can inhibit the activity and proliferation of granule cells,induce cell apoptosis.4.High expression of TIPE1 can promote the expression of caspases(3,8 and 9)in ovarian cancer cells and granulosa cells,and promote cell apoptosis.Conclusion:1.TIPE1 can be used as a potential marker for predicting the poor prognosis of patients with ovarian cancer.2.TIPE1 inhibits the proliferation of ovarian cancer cells and granulosa cells by promoting the expression of caspases.