| Purpose:We took the DN rat model, built with HG & HF feeding and once injection through vena caudalis with STZ as the research object, to investigate the pathological mechanism of DN in early period of occurrence and development and to approach the effect and acting mechanism of pescription of invigorate vital energy, detoxicating and activating collaterals (IDAP) in preventing and curing DN by evaluating the general condition of rats, detecting the related figure such as weight, BFS, glycosylated hemoglobin, uria albumin, serum beta 2-microglobulin and MCP-1 and TNF-?in nephridial tissue and observing the pathological change of kidney.Material and method:1.MaterialsWe took healthy and male, SD rats in SPF Level as research object. Pescription of invigorate vital energy, detoxicating and activating collaterals (IDAP) was composed of the detocting free powder of herbs including solomonseal rhizome, astragali,radix, coptis chinensis, giant knotweed rhizome, eupatorium and sanguisuge. benazepril hydrochloride pellet was taken as the positive controlling factor.2.Grouping dividing and DN rat model building:72 SD rats were randomly divided into 6 groups as following, control group (A), blank group (B), LD group (D1), HD group (D2) and positive control group (E). We built up DN rat model by 4-week HG & HF feeding and once injection through vena caudalis with STZ and recognized the model by the standard of FBG?16.7mmol/L.3.MedicationWe daily intragastricly administrated the rats of Group A and B with NS 2ml, the rats of Group C, D1, and D2 with IDAP, drug granules dissolved in distilled water, at respectively dose as 14.7g/kg, 14.7g/kg and 44.1g/kg and the rats of group E with Benazepril Hydrochloride Tablets at dose of 1.05mg/kg. The procedure administrating with drug lasted 4weeks in total.4.Figure and Assay methodThe pharmacodynamics figure we took to observe the effect and mechanism of IDAP includes general condition, weight, blood glucose, serum beta2-microglobulin, uria-albumin (24h), pathomorphism change of nephridial tissue, hs-CRP and ADPN in serum (ELISA), MCP-1 and TNF-?.5.Statistics analysisMeasurement data was taken by using SPSS11.5 software and P<0.05 was recognized as significant difference existing.Results:1.Influences to general conditions of rats:Group A- obviously weight gained, in good mood and sensitive, agile moving, tramosericeous color pattern and no death.Group B- polydipsia, polyphagia, hyperdiuresis, depressed, dull, sluggishly moving, dry and rare fur, bending, tow rats got ulcerate in tail.Group C, D1, D2, E– similar manifestation to Group B but less in degree.2.Influences to weight: Compared to group A, result of group B is significantly lower when 4w after pattern achieved (P<0.01). Compared to group B, result of group C, D1, D2, E is significantly higher (P<0.01).3.Influences to blood glucose: 4w after pattern achieved, result of group B is significantly higher than group A (P<0.01). Compared to group B, result of group C, D1, D2, E is significantly lower (P<0.01).4.Influences to glycosylated hemoglobin: Result of group B is significantly higher than group A, interclass significant difference shown (P<0.01). Compared to group B, results of group C, D1, D2, E are all significantly lower (P<0.01).5.influences to results of uria-albumin (24h): Result of group B is significantly higher than group A, interclass significant difference shown(P<0.01). Compared to group B, results of group C, D1, D2, E are all significantly lower (P<0.01).6.Influences to Beta 2-Microglobulin in serum: Result of group B is significantly higher than group A, significant difference shown(P<0.01). Compared to group B, results of group C, D1, D2, E are all significantly lower (P<0.01).7.Influence to nephric pathological change: Observed with light microscope, nephridial tissue of group A appears normal acinus renis, no cellular proliferation, tight mesangial region, open capillary lumen, RBC suffusion. In contrast, nephridial tissue of group B appears accretion between capsula glomeruli and loop of micrangium, cortical substance narrowed, vacuolus-like change in endepidermis of nephric tubule, protein-like mas in lumina, nephric tubule ruined and cellula epithelialis nucleus partly disappeared. Nephridial tissue of group C, D1, D2 and E are all observed better than group B and all the pathological changes got improved. We can see abatement in acinus renis partly accreted, cort narrowed, vacuolus-like change in endepidermis of nephric tubule and mesangial cellular proliferation.8.Influences to serum ADPN: Result of group B is significantly lower than group A, significant difference shown(P<0.01). Compared to group B, results of group C, D1, D2, E show significantly upgrading tendency (P<0.01).9.Influences to hs-CRP in serum: Result of group B is significantly higher than group A, significant difference shown(P<0.01). Compared to group B, results of group C, D1, D2, E show significantly descending tendency (P<0.01).10.Influence to MCP-1 in nephridial tissue:A. m-RNA content: Result of group B is significantly higher than group A, significant difference shown (P<0.01). Compared to group B, results of group C, D1, D2, E show significantly descending tendency (P<0.01). B. Protein expression of MCP-1:Result of group B is significantly higher than group A, significant difference shown (P<0.01). Compared to group B, results of group C, D1, D2, E show significantly decreased (P<0.01) 11.Influence to TNF-?:A. m-RNA content: Result of group B is significantly higher than group A, significant difference shown (P<0.01). Compared to group B, results of group C, D1, D2, E show significantly descending tendency (P<0.01)B. Protein expression of TNF-?:Result of group B is significantly higher than group A, significant difference shown (P<0.01). Compared to group B, results of group C, D1, D2, E show significantly decreased (P<0.01).Conclusion:1.Pescription of invigorate vital energy, detoxicating and activating collaterals (IDAP) can obviously regulate and amend the pharmacodynamics figure of DN rats, including general condition, weight, blood glucose, glycosylated hemoglobin, 24h-urina mALB,?2- microglobulin in serum and patho-change in nephridial tissue, meanwhile IDAP can control the BG and protect nephridial tissue.2.Pescription of invigorate vital energy, detoxicating and activating collaterals (IDAP) can obviously regulate and amend the figure of drug action as ADPN, hs-CRP, MCP-1 and TNF-?. IDAP can control the inflammatory reaction during the occurrence and development of DN and its mechanism maybe take the way of regulating the above figures.3.Decrease in ADPN expression, increase in hs-CRP content and increase in expression of MCP-1 and TNF-?can be checked in the DN rat, which indicates that the mechanism in the occurrence and development of DN in early period maybe related to the abnormal change of the above figures. |
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