Study On Systems Bilogy For The Mechanism Of TCM Shuanglong Formula

This paper studied Shuanglong Formula (SLF), a kind of traditional Chinese medicine, through systems biology integrated with chemomics. The aim of this paper was to systematically discuss the mechanism and pharmacodynamic molecular basis of SLF, and to offer the reference of technology for related investigation of other TCM formula.First of all, on the rat bone marrow mesenchymal stem cells (MSCs) differentiation model, the cardiac-specific proteins were identified using immunofluorescence staining method to evaluate the effects of SLF on MSCs differentiation. And then differentially expressed proteins were screened during the process of MSCs differentiation induced by SLF. Finally, 13 differentially expressed proteins were successfully identified. During the process of MSCs differentiation, eEf-2, Hspa8, Nme2?PKM2 and S100A11 protein played an important role. They might direct MSCs differentiation through regulated PI3K signaling pathway, MAPK signaling pathway and calcium ion channels.Secondly, a hypoxia/reoxygenation (H/R) myocardium model was established. The protective effect of SLF against H/R injury was investigated in this model using MTT method. Then the active ingredients, which existed inside cells, were screened and determined using HPLC-MS. The MTT staining results showed that SLF can improve cell survival up to 62%. It illustrated that SLF was effective in protecting cultured cardiomyocytes against H/R injury. The active ingredients mainly included Re, Rg1, Rf, Rb1 and Rd, all which had significant antioxidant to achieve by adjusting the calcium ion channel.Thirdly, the rat model of myocardial infarction induced by ligation of coronary artery was established. After treatment by SLF, differential expressed genes were screened by cDNA microarray to investigate the molecular mechanism of SLF. The results showed that differential expressed gene mainly related MAPK pathway and the basic excision repair between the SLF group and the model group. So, SLF may regulate MAPK pathway and the basic excision repair process to achieve the effect of reducing myocardial injury and protecting cardiac function?Finally, using the rat model of myocardial infarction and cDNA microarray technology, the mechanism of several kinds TCM was investigated. SLF, heart-protecting musk pill and Danhong injection all have effect on treatment myocardial infarction. The gene expression changes were observed and differentially expressed genes were screened from the chip data combined with all group. The results showed that 9 genes had very important roles, such as Cfb, Nfil3, Arntl, Nppa and so on. They may be the common target genes of TCM treatment of myocardial infarction and provided important clues for future research.