The Action Mechanims Of Traditional Chinese Medicine Formula Dihuangyinzi For The Treatment Of Stroke And Its Sequelae

Stroke is an acute cerebrovascular disease,due to lack of blood flow to the brain results in cell death.Traditional Chinese Medicine have long been shown effective in treating stroke and its sequelae.Here we proposed a systems pharmacology approach using a clinically effective herb formula Dihuangyinzi(DHYZ)as a tool to detect the molecular mechanisms for the treatment of stroke and its sequelae.All ingredients of DHYZ(15 herbs)were manually collected from references or downloaded from the traditional Chinese medicine systems pharmacology database and analysis platform.The pharmacological system generated 92 bioactive ingredients from the Chinese herbal.We collected and analized the genes related to DHYZ and stroke by literature mining and databases.and then these genes were mapped into the PPI(PPI,Protein-Protein Interation)network anlysis.There was a significant correlation between genes of two sets of genes in the PPI network,which suggest that the close correlations of genes for DHYZ and stroke.Then drug targets were obtained via weighted ensemble similarity(WES)model.We evaluate whether two drug targets can have synergistic effect based on pathway information and generated 138 and 43 synergistic and antagonistic relationships respectively.Taken together,the compounds-targets network,targets-pathways network and targets synergism antagonism network were constructed.The present work has provided an integrated systems pharmacology framework for the therapeutic mechanism of DHYZ on stroke and its sequelae,which will also facilitate the application of traditional Chinese medicine in modern medicine.