| Objective Proteomics methods are applied to detect and identify the serum difference proteomes between ST segment elevation acute myocardial infarction(STEMI) patients and health persons.To explore the proteomes that may related to acute myocardial infarction will be benefit for the diagnose,the explain of pathogenesy and the medicine treatment of STEMI.Metheds 13 STEMI patients' and 26 health persons' serum were collected. Immobilized pH gradient two-dimensional polyacrylamide gel electrophoresis (2-DE),PDQuest 2DE analysis software,peptide mass fingerprint based on matrix-assisted laser desorption/ionization time of flying mass spectrometry(MALDI-MS/MS),and NCBInr database searching were applied to detect and identify the difference serum proteomes.Result Eight differential protein spots were detected and five proteins, including Fibrinβ,fibrinogenγ,CD5L,apolipoprotein A-Ⅳandα 2macroglobulin were identified. The level of Fibrinβ,fibrinogenγ,CD5L are up-regulated express and apolipoprotein A-Ⅳ,α2macroglobulin are down-regulated express in STEMI patients'serum compared with health persons' serum. Fibrinβ,fibrinogenγare the related products of Fibrinogen.Conclusion The results indicate that Fibrinogen and CD5L may promote the development of STEMI to some extent. On the contrast,apolipoprotein A-Ⅳandα2macroglobulin would protect against the development of STEMI. Monitoring the serum Fg level of patients suffered CHD would help to the precaution of STEMI,and,to aim directly at the treatment of Fg would also help to realize the turnover of CHD.Besides,the formation and the development of STEMI may be relate to the disorder of lipoid metabolism and the disequilibrium of immunoregulation.
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