The Discussion On The Astragalus Injection Of The Mechanism Of Lung Cancer Mouse Model

Objective:Froming the lungs the main gas theory, this thesis expounds the mechanism of lung cancer a systematic explanation and demonstration in order to enrich the prevention and treatment of lung cancer. Through the establishment of mouse Lewis lung cancer model. Observation of Astragalus injection on survival time of mice, mice tumor weight and inhibition rate of metastasis of lung cancer in mice. Immunohistochemical method used to observe the astragalus on the mouse tumor necrosis factor (TNF-a) and matrix metalloproteinase -9 (MMP-9) impact. From the micro-mechanism of Astragalus injection on the suppression of lung cancer in mice and transfer mechanism of action with a view to effective clinical use of astragalus to provide experimental basis for the treatment of lung cancer.Methods:To establish a mouse Lewis lung metastasis model, using Chinese and Western medicines for treatment compared to observe the astragalus on the impact of tumor tissue in mice. By observing the general situation in mice, weight change, inhibition rate, the lung tissue matrix metalloproteinase MMP-9 and tumor necrosis factor TNF-a and other cytokines. From the cellular and molecular level to explain the mechanism of these changes and explore the role of Astragalus injection on the relevance of lung cancer for the prevention and treatment of lung cancer to provide new ideas and theoretical basis.Results:The mouse Lewis lung metastasis model of lung cancer in line with the clinical performance of general clinical symptoms of lung cancer and consistent with changes in the indicators also consistent with changes in indicators of lung cancer. Astragalus injection on mice transplanted lung cancer and pulmonary metastasis in mice were significantly inhibited (P<0.05), and astragalus injection on immune function significantly improved. Astragalus also reduce TNF-?and MMP-9 protein expression (P <0.05).Conclusion:Astragalus injection has obvious inhibitory effect on mouse lung cancer; can enhance immune function of lung cancer in mice, showing that the occurrence of Astragalus injection in mice, and metastasis of lung cancer plays an important role in the intervention. Astragalus injection can significantly reduce the tumor necrosis factor (TNF-?) and matrix metalloproteinase -9 expression levels, indicating a certain degree of Astragalus injection in prevention and treatment of lung cancer can be.