Changes in cell surface glycoproteins associated with resistance to concanavalin A in a human erythroleukemia cell line (K562)

y the methods of gel electrophoresis, protein blotting, and lectin staining, we compared the cell membrane glycoproteins of the human erythroleukemia cell line K562 with those of a NK-resistant subclone (Clone I), and a Con A-resistant subclone of Clone I called "Con A-R1". Although parental K562 and Clone I cells gave identical patterns, Con A-R1 contained two major populations of fucosylated proteins. Immunological analysis by flow cytometry and histochemical staining with lectins demonstrated that in Con A-R1 the H blood group epitope appeared concurrently with the disappearance of the...