Leukemic Cell K562 And Enhancement Of The Sensitivity To Chemotherapy

Leukemia is one of 10 cancers threathening people's health, which is the first one in ten-age people. Chemotherapy is still major method, but toxicity to normal cells and resistance to multidrugs influence therapeutic effect. Recently,cytobiology progress made people realize that apoptosis plays an important role in oncogenesis and advance.Decrease in apoptosis threshold and increase in cell apoptosis are important methods for improving clinic effect. Higher expression of inhibitors of apoptosis protein(IAP) ,which has been identified in human body, is one of the reasons for escaping anticancer drugs. In IAP family, survivin selectly expresses in tumor tissue, lacks of expression in normal adult tissue, which made survivin turn into a therapeutic target. In this study the expression of survivin gene was blocked by its antisense oligodeoxynucleotide(ASODN),which inhibited tumor cells' proliferation, increases tumor cells apoptosis aiming at enhancing chemotherapy sensitivity.Part I. Effect of Survivin antisense oligodeoxynucleotide on inhibition rate and chemotherapy sensitivity of leukemic cell K562Objective: To explore the effect of ASODN targeting survivin on inhibition rate and chemotherapy sensitivity of leukemic cell K562. Methods: Survivin ASODN was synthesized and transfected into K562 cells by liposome (Lip). MTT assay was used to detect cytotoxicity of...