| The lens capsule consists of a dynamic matrix of molecules providing the lens with structural support as well as a selectively permeable barrier to the surrounding eye tissues. The main structural element of the capsule is a scaffolding of collagen IV on which other proteins such as laminin, nidogen, and heparan sulfate proteoglycans bind to create a strong meshwork. This meshwork is extremely thick, the anterior pole measuring over 25im in humans and approximately 10im in mice, and provides the capsule with the strength to transmit radial tension from the cilary apparatus required for primate lens accommodation. The capsule meshwork also contains many charged and hydrophobic regions which, along with pore size, contribute to the selective permeabilty attributed to it.;My research defines the factors responsible for the thickness and behavior of proteins diffusing in the lens capsule. I show that the mouse lens capsule is an ideal model for studying the properties of basement membranes. I have developed relatively uninvasive techniques to more accurately measure the thickness of the capsule as well as describe the behavior of proteins diffusing within it. The data gained using these techniques can be useful in developing new strategies to fight diseases involving basement membranes. |
