Correlation Between Sarcopenia And Metabolic Syndrome In Middle-aged And Elderly People And Metabonomics Research

Objective:The analysis of the elderly in urumqi to the content of fat weight,muscle mass,distribution characteristics and the relationship with obesity,on the basis of discussing reduce muscle disease and diagnostic parameters(muscle mass,muscle strength and physical function)associated with the metabolic syndrome and components,and find the related to reduce muscle disease metabolites and metabolic pathways,and to reduce the early recognition and diagnosis of muscle disease provide candidate metabolic markers Methods:1)Using the cross-sectional survey method,from July 2018 to January 2019,the national key research and development plan "Northwestern Region Natural Population Cohort Study" sub-project "Xinjiang Multi-ethnic Natural Population Cohort Construction and Health Follow-up Study" 1924 40-year-old people at the Urumqi site The above community residents are the research objects,and the participants who meet the inclusion and exclusion criteria will be subjected to questionnaire survey,physical examination,body composition measurement,blood sample collection and testing.2)Taking community residents over 50 years of age as the research objects,collecting dietary nutrient intake and physical activity information,measuring grip strength and 4m pace.Multivariate ogistics regression model was used to analyze the association of sarcopenia and diagnostic parameters with metabolic syndrome and its components by gender.3)The case group selected 40 sarcopenia patients,and the control group selected 40 healthy people with the same gender,age±1 year,and height±2cm.UHPLC-QTOFMETS technology was used to detect the small molecule metabolites in the serum of sarcopenia patients and healthy controls,and the non-targeted metabonomics method was used to determine the difference in muscle loss Disease-related new metabolites and metabolic pathways.Results:1)This study collected 1961 baseline data and excluded 37 people with incomplete basic information.Finally,1924 middle-aged and elderly residents were included in the body composition analysis.2)The body water,minerals,protein,and basal metabolic rate of the whole population and men of all ages were higher than those of women(P<0.001).The body water,mineral,protein,and basal metabolic rate of men and women show a downward trend with age(P<0.001).3)The FM and FMI of the whole population and men of all ages were lower than those of women in the whole body,trunk and limbs(P<0.001).The male PBF gradually increased with age,and the PBF of males 60 and 70 years old and above was higher than that of the 40 and 50 years old(P<0.01).Women's PBF,trunk and limbs FFMI increased first and then decreased with age,reaching a peak at the age of 60.The PBF,trunk and limbs FFMI of women in the 40-year-old age group were lower than those in the 50-year-old,60-year-old,and 70-year-old age group(P<0.01).The FFMI of trunk and limbs did not change with age in men.4)The effect of age and sex on lean body mass and skeletal muscle mass:?The FFM,FFMI,and lean body mass percentages of the whole population and men of all ages were higher than those of women(P<0.001).The FFM and FFMI of both men and women showed a downward trend with age(P<0.001).The lean body mass of men's body,trunk and limbs decreased faster than women's.?The SMM and MI of men are higher than those of women in the whole population and all age groups(P<0.001),and the ASM,ASM/ht2,ASM/wt,and ASM/BMI of men are also higher in the whole population and all age groups.Female(P<0.001).Both male and female SMM and MI showed a downward trend with age(P<0.001).The ASM,ASM/ht2,ASM/wt,and ASM/BMI of males and females all showed a downward trend with age(P<0.001),and the skeletal muscle mass of male limbs decreased faster than females.? The LCMItotal and eLCMIregional of the whole population and men of all ages were lower than that of women(P<0.001),and the LCMItotal and eLCMIregional of men and women showed an upward trend with age(P<0.001),and the increase rate of women was faster than that of men.5)The relationship between obesity and lean body mass and skeletal muscle mass:?With the increase of BMI grade level,FFM,FFMI,SMM,MI and ASM of the whole body,trunk and limbs showed an upward trend(P<0.001),while the percentage of lean body mass showed a downward trend(P<0.001).With the increase of BMI level,the order of segmental lean body weight increase is:upper limb>trunk>lower limb.After controlling for gender,age and weight,with the increase of BMI grade level,ASM/ht2 increased,while ASM/wt and ASM/BMI gradually decreased.?The whole body and segmental FM,FMI,SMM,MI and ASM of central obesity are higher than those of non-central obesity,but the percentage of lean body mass of central obesity is lower than that of non-central obesity.After controlling for sex,age and weight,compared with non-central obesity,central obesity increased ASM/ht2,and ASM/wt decreased,while ASM/BMI was not related to central obesity.6)The detection rate of sarcopenia among community residents over 50 years old in Urumqi is 13.49%.Among them,the male detection rate was 15.56%,and the female detection rate was 12.12%.With the increase of age,the detection rate of sarcopenia in men and women is on the rise.In the age group over 70 years old,27.83%of men and 31.28%of women.7)The relationship between sarcopenia and metabolic syndrome and its components.Male sarcopenia increases the risk of metabolic syndrome and hyperglycemia,but reduces the risk of central obesity.The OR(95%CI)is METS(OR=2.09,95%CI:1.07-4.11);Obesity(OR=0.47,95%CI:0.26-0.74);hyperglycemia(OR=2.31.95%CI:1.25-4.27)Female sarcopenia increases the risk of metabolic syndrome,hyperglycemia,and hypertension.The OR(95%CI)is METS(OR=1.47.95%CI:1.02-3.03),hyperglycemia(OR=1.61,95%CI:1.01-2.55),hypertension(OR=1.66,95%CI:1.10-2.49).There was no association between sarcopenia,hypertriglyceridemia,and low HDL-C in men and women.8)The relationship between the diagnostic parameters of sarcopenia and the metabolic syndrome and its components:?In men,with the increase of ASM/wt level,the risk of central obesity and hypertension gradually decreased(trend P<0.001),the OR of the risk of central obesity in different quantile groups of ASM/wt(95%CI)respectively:1,0.65(0.47,0.98),0.62(0.43,0.93),0.60(0.38,0.89).The OR(95%CI)of the risk of hypertension in the different quantiles of ASM/wt were:1,0.70(0.44,0.96),0.68(0.48,0.96),0.62(0.46,0.92).In women,with the increase of ASM/wt level,the risk of metabolic syndrome and hyperglycemia gradually decreases(trend/P<0.001).Compared with the Q1 quantile array of ASM/wt,the Q3 quantile array(OR=0.56,95%CI:0.34-0.94)and the Q4 quantile array(OR=0.42,95%CI:0.23-0.76)found that women' s metabolic synthesis The prevalence of symptoMetS decreased.The OR(95%CI)of the risk of hyperglycemia in different quantile groups of ASM/wt were:1,0.45(0.28,0.73),0.53(0.31,0.90),0.49(0.27,0.90).There was no association between ASM/wt and hyperTGemia and low HDL-C in men and women.?In men,with the increase in grip strength,the risk of metabolic syndrome,central obesity,hypertension,hyperglycemia and hypertriglyceridemia gradually decreased(trend P<0.001).Among them,the ORs(95%CI)of central obesity in different quantiles of male grip strength were:1,0.97(0.73,1.48),0.93(0.65,1.86),0.51(0.30,0.87);OR of hypertension(95%)CI)are:1,0.88(0.53,1.45),0.63(0.38,1.04),0.49(0.31,0.79),0.58(0.35,0.95).In women,grip strength is associated with the risk of metabolic syndrome,hypertension,and hypoHDL-Cemia.The risk of female metabolic syndrome decreases with the increase of grip strength(trend P<0.001),but the risk of hypertension and low HDL-Cemia is not related to grip strength(trend P>0.05)).Compared with the Q1 quantile of grip strength,the prevalence of hypertension in the Q3 quantile decreased(OR=0.61,95%CI:0.38-0.97),and in the Q4 quantile(OR=0.36,95%CI:0.21-0.60)and hypoHDL-Cemia(OR=0.45,95%CI:0.22-0.89)prevalence decreased.?In men,with the increase in pace level,the risk of hyperglycemia gradually decreases(trend P<0.001),and the OR(95%CI)of the risk of hyperglycemia in different quantile groups of male pace They are:1,0.72(0.45,0.99),0.56(0.34,0.91),0.49(0.31,0.77).There is a correlation between pace and hypertension,but there is no linear relationship between the two(trend P>0.05).Compared with the Q1 quantile of pace,the prevalence of hypertension in the Q4 quantile decreases(OR=0.59,95%CI:0.37-0.96).In women,the prevalence of metabolic syndrome and central obesity are related to pace(trend P>0.05).Relative to the Q1 quantile of pace,the metabolic syndrome is in the Q4 quantile(OR=0.54,The prevalence of 95%CI:0.37-0.96)and central obesity(OR=0.54,95%CI:0.34-0.87)decreased.With the increase of stride level,the prevalence of hyperTGemia gradually decreased.The OR(95%CD of the risk of hyperTGemia in different quantiles of stride speed were:1,0.63(0.42,0.93)),0.63(0.42,0.94),0.49(0.32,0.75).There was no association between low HDL-C and pace in both men and women.9)Through non-targeted metabolomics analysis,it was identified that the content of 12 compounds was significantly increased in the sarcopenia group,and 7 compounds were significantly decreased in the sarcopenia group.The top three compounds in the sarcopenia group are:indole sulfate,3-chloro-L-tyrosine and 2-deoxyribose-5-phosphate;the top two metabolites in the sarcopenia group are:1-Naphthalenediacetic acid and thiamine monophosphate.Through the ROC curve evaluation of different metabolites,10 compounds with AUC>0.7 were screened out.In the sarcopenia group,the five compounds with higher metabolite content than the healthy control group are:1-palmitoyl-2-oleoylphosphatidylglycerol,nervonic acid,4,4'-[(1-methylethyl)di Sulfur]bis[(2,6-di-tert-butyl)phenol],protoporphyrin IX and tricosanoic acid;the content in the sarcopenia group is lower than that in the healthy control group.3 compounds are:4-nitrophenol,Trehalose and 1-naphthyridine acetic acid.Conclusion:(1)to systemic and segmental fat weight,body and limbs skeletal muscle quality is given priority to gender differences in body composition,and male and female skeletal muscle mass were declined as the growth of the age,compared with the ASM/ht2,ASM/BMI,ASM/wt closer relations with metabolic risk factors,more accurately identify obesity overweight reduces the muscle quality.2)Muscular dystrophy in the middle-aged and elderly people in Urumqi is associated with MetS and components,and there is a gender difference.Prevention and early identification of the occurrence of muscular dystrophy in the middle-aged and elderly people is of great significance for the prevention and control of metabolic syndrome,diabetes and cardiovascular and cerebrovascular diseases.3)In this study,19 kinds of differential metabolites related to sarcosiosis were identified,mainly involving the metabolic pathways of amino acids and fatty acids.Subsequent clinical and target verification can be further carried out to explore reliable specific metabolic markers.