| Objective:1.Through this part of the clinical data of 120 cases of patients with gastric carcinoma were collected,The relationships between mannose receptor expression and clinicopathological features of gastric cancer patients were analyzed.Prognostic value of mannose receptor ingastric cancer patients.2.In second study,the expression of miR-1260 b and its relationship with clinicopathological features in 120 patients with CRC were studied,To analyze the relationship between miR-1260 b and the survival time of patients with colorectal cancer,and to evaluate the value of clinical prognosis.Method: 1.Immunohistochemistry was performed on 120 cases of gastric cancer and corresponding paracarcinoma tissue.Determination of mannose receptor expression by RT-PCR.The patients were divided into two groups according to the intensity of staining.Low expression group and high expression group of mannose receptor.Chi square test was used to analyze the relationship between mannose receptor expression and clinicopathological parameters.Kaplan-Meier method and Log-rank test was used for survival analysis and to compare the survival difference between the two groups.Overall survival(OS)and disease-free survival(DFS)were the main outcome.The relationship between the expression of mannose receptor and prognosis was analyzed by univariate and multivariate COX regression models.2.The expressions of miR-1260 b was detected by real-time quantitative polymerase chain reaction(Real-time PCR)in 120 cases of CRC tissues.The correlation of miR-1260 b expression with the clinicopathologic features of CRC was analyzed by SPSS 21.0statistical software.The Kaplan-Meier method was used for survival analysis.Cox regression analyses were conducted to determine whether miR-1260 b was an independent predictor of survival for CRC patients.Results: part1 The prognostic value of mannose receptor in gastric carcinoma.1.Expression of mannose receptor in gastric carcinoma.The patients were divided into two groups according to the intensity of staining:mannose receptor lowexpression group and mannose receptor high expression group.The expression of mannose receptor protein in gastric cancer cells was up to 45.8%,significantlyhigher than that in paracarcinoma tissue 20%(x2=6.286,P=0.012).In order to further verify the results of immunohistochemistry,the expression level of mannose receptor mRNA was detected by RT-PCR.30 patients were randomly selected to take gastric cancer tissues and corresponding adjacent tissues.19 of the 30 pairs of samples showed that the expression of mannose receptor mRNA in gastric cancer tissue was increased by more than 2 times,and the highest increase of,maximum increase of 14.7 times.2.The relationship in patients with gastric cancer between mannose receptor and clinicopathological characteristics.High expression of mannose receptor was closely related to tumor size,T stage,N stage,and UICC stage of gastric cancer(P<0.05).The age and sex of patients with gastric cancer,M staging,tumor location,Lauren classification,lymphatic invasion,and postoperative chemotherapy and mannose receptor expressionThere was no statistical significance(P>0.05).3.Mannose receptor expression and prognosis in patients with gastric cancer.The Kaplan-Meier survival model indicated that the survival of patients in the high-expression mannose receptor group was significantly shorter than in the low-expression mannose receptor group(P<0.05).Cox regression analysis showed that high mannose receptor expression was an independent predictor for the prognosis of patients with gastric cancer.part2 The prognostic value of miRNA-1260 b in colorectal cancer.1.miR-1260 b and CRC.The miR-1260 b expression in CRC was significantly higher than the expression levels in the corresponding para-carcinoma tissues(P<0.001).To explore the relationship of miR-1260 b expression level,the expression level of mi R-1260 b in patients with different lymph node status was analyzed.Compared with patients without lymph node metastasis,the miR-1260 b expression levels with lymph node metastasis increased significantly in CRC tissues(P<0.001).2.Expression of miR-1260 b and clinicopathological features of CRC.According to the expression levels of miR-1260b(2-??Ct median value as the cut-off value),120 cases of CRC patients were classified into high expression group of the miR-1260 b and low expression group.Specifically,67 patients with CRC were in the high expression group,and the other 53 were in the low expression group.We analyzed the relationship between clinicopathological features of CRC patients and miR-1260 b expression.The high expression levels of miR-1260 b in CRC patients was associated with lymph node metastasisand venous invasion(P<0.05).However,the high miR-1260 b expressionhad no significant correlation with other clinical parameters.3.Clinicopathological factors of miR-1260 b expression and CRC patient prognosis.In order to analyze the relationship of miR-1260 b expression levels between and prognosis of patient with CRCs,we plotted the survival curves(DFS and OS)of all patients by Kaplan-Meier method.The results suggested that the CRC patients with high miR-1260 b expression levels had significantly shorter survival rates than low miR-1260 b expression patients.COX analysis univariate analysis showed that venous invasion(P<0.05),depth of invasion(P<0.001),lymph node metastasis(P<0.001)and high miR-1260 b expression levels(P=0.003)were significantly correlated with OS.Multivariate analysis using the Cox regression model suggested that high miR-1260 b expression was an independent indicator of poor patient prognosis.Conclusions:1.By mannose receptor in gastric cancer high expression and clinical pathological features,we speculate that may be closely related to the occurrence and development of gastric cancer,the expression was positively related to malignant degree of gastric cancer with mannose receptor.The survival time of patients with high mannose receptor expression was shorter than that of patients with low expression of mannose receptor.Multivariate regression analysis showed that high expression of mannose receptor protein is a potential tumor marker of poor prognosis in patients with gastric cancer.Is an independent indicator of poor prognosis.2.We found that in colorectal cancer tissues and lymph node metastasis in patients with high expression of miR-1260 b,and combining with the analysis and the relationship between the clinical and pathological features,found with lymph node metastasis and venous invasion were statistically significant,which means that miR-1260 b may promote early metastasis of colorectal cancer.Patients with high miR-1260 b expression had shorter survival time,using COX regression analysis.There is a correlation between miR-1260 b and survival time of two single factor,multivariate regression analysis,shows that the high expression of miR-1260 b is a potential tumor marker of poor prognosis in patients,is an independent index. |
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