| Uterine leiomyoma is one common benign tumor in women and threaten women health. Its common symptoms are abnormal uterine bleeding, pelvic oppress, pain. It is related with hormones, genital meatus inflammation and environmental factors which play the important roles in development and progress of uterine leiomyoma. Its pathology are proliferation of spindle type muscle cell and fiber connective tissue, increased expressions of receptors of hormones, plentiful extracellular matrix (ECM), as well as infiltration of inflammatory cells. The pathogenesis of uterine leiomyoma is unclear, the relationships among inflammation, hormones, fibrosis are not determinated.The aim of this study is to research the role of inflammation in development of uterine leiomyoma and to explore relationships between inflammation and fibrosis or hormones.Interleukin-17(IL-17) is proinflammatory cytokine produced from T cell, an initiating factor of inflammation induced by T cells in early time and is closely involved in inflammation. However, the study on expression of IL-17 in uterine leiomyoma has not been reported.In the present study, the mRNA levels of I1-17, estrogen receptor (ER), progestogen receptor (PR), vitamin D receptor (VDR), interleukin-1? (IL-1?), cluster of differentiation 3(CD3), CD28, C-X-C chemokine ligand 6(CXCL6), T-box expressed in T cells (T-bet), GATA-binding protein-3 (GATA-3), factor forkhead box protein 3 (FOXP3), matrix metalloproteinase 9 (MMP9), transforming-growth factor-beta 3 (TGF-?3) were examined by using quantitive Real Time-PCR. The difference between the expressions of these molecules in leiomyomas and the matched normal myometrium was compared, at last the changes were determined. Furthermore, the relationships among these changed factors in uterine leiomymas were analyzed by analysis of pearson. The roles and interactions of these factors in pathogenesis of uterine leiomyomas were discussed.The results were shwed following:1) The mRNA levels of IL-17, CD3, CXCL6, T-bet, GATA-3 and FOXP3 were increased significantly in leiomyomas when compared with the matched normal myometrium, CD28 mRNA level was not changed.2) The mRNA level of IL-1? was increased in leiomyomas when compared with the matched normal myometrium, but threre was not significant difference.3) The mRNA levels of MMP9 and TGF-?3 were increased significantly in leiomyomas when compared with the matched normal myometrium.4) The mRNA levels of ER1 and ER2 were increased significantly in leiomyomas when compared with the matched normal myometrium. The mRNA levels of PR and VDR were increased, but there was not significant difference. The estradiol (E2) level was increased in peripheral blood in patients with leiomyomas when compared with normal group, but there was not significant difference. The progesterone (P) was not increased in peripheral blood in patients with leiomyomas when compared with normal group.5). The results of analysis of Pearson:The expression of I1-17 is positively related with CD3 expression. The expression of I1-17 is positively related with CXCL6 expression. The expression of I1-17 is positively related with expressions of T-bet, GATA3, FOXP3. The expression of I1-17 is positively related with expressions of TGF-?3, MMP9. The expression of MMP9 is positively related with the expression of TGF-?3. The expression of IL-17 is not related with expressions of ER1 and ER2. The expression of TGF-?3 is positively related with expressions of ER1 and ER2. The expression of MMP9 is positively related with expressions of ER1 and ER2. All above results showed that:the increases of inflammatory factors IL-17, CD3, CXCL6 and transcription factors T-bet?GATA3, FOXP3 in leiomyomas are involved in inflammation in leiomyomas. The relationships between IL-17 and FOXP3, T-bet, GAT A3 suggests that FOXP3, T-bet, GAT A3 regulate the expression of IL-17. The relationship between I1-17 and MMP9, TGF-?3 suggests that IL-17 activates MMP9, TGF-?3 and further induces fibrosis of uterine muscle cells and the extracellular matrix increase, finally results in leiomyomas. Leiomyomas is estrogen depdent tumor, the results showed that there was not relationship between TL-17 and ER1, ER2. There was the relationship between ER and TGF-?3, MMP9. The estrogen binds to estrogen receptor and activates TGF-p3, MMP9, then induces mitosis and proliferation of uterine muscle cells, increases of synthesis and secretion, as well as enhances the progress of Fibrosis.Thus, development of uterine leiomyma is related with increases of inflammation, fibrosis and estrogen. IL-17 is associated with fibrosis of uterine muscle cells and promotes the progress of fibrosis. The studies on IL-17 and ER will provide a new insight for treatment of uterine leiomyma and play an important role in early treating and improving prognosis on uterine leiomyma. |
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