| ObjectiveAging is the result of the interaction of genetic, internal andexternal factors. It happens morphologically and functionally inthe law of life. The cognitive function will decline with aging inboth human beings and animals. What’s more, the Yang-deficiencysymptom will accompany with aging. This study focus on the learningand stduy decline in the aging model rats with Yang-deficiency, andhave a treatment with Shuanggu Yitong elEACtro-acupuncture to rats,it is to explore the efficacy Shuanggu Yitong acupuncture methodimproving the learning and study decline, and try to providescinetific evidence for the treatment how to control the Long-TermPotentiation in the Hippocampus.MethodsForty Sprague Dawley rats were randomly divided into fourgroups:Young Group(Y),Model Group,and electro-acupuncture controlGroup (EAC)，Shuanggu Yitong electro acupuncture Group (EA). Therats distributed in Model Group, EA Group, and EAC Group wereinjected with D-galactose subcutaneously for40days andhydrocortisone for7days in muscles of the rat’s left leg, thiscould induce the Yang-deficiency symptom in aging model rats. The rats in Young Group were injected of same amount of saline at sametime. Rats in EA Group were treated with Shuanggu Yitongelectro-acupuncture（Guanyuan,Zusanli,Baihui）, and EAC group werestimulated with compaired electro-acupuncture therapy(Zhongji,Yinlingquan,Yintang), each treatment were last for4weeks.Finally,Enzyme linked immunosorbent assay（ELISA）are used to detEACt thequality of cAMP and cGMP and the percentage of cAMP/cGMP, Morriswater-maze task was used as the judging critera for learning andmemory behavior; Synaptic structural parameters were analysised byelEACtron microscopy and biological image analysis system;Population spike was chEACked to detect the changes of potentialamplitude induced by the high frequency stimulation (HFS); Contentof BDNF was detected by the radioimmunity method;PKC mRNAexpression was examined through the RT-PCR;the expressiondifference of NMDA receptor also was checked through Western blotmethod.Results1.The quality of cAMP and percentage of cAMP/cGMP in Model ratswere lower than the Young group and EA group and EAC group, thequality of cGMP bEACame higher on the contrary.there weresignificant difference between them（P＜0.01）.The model rats showeda series of asthenic symptom such as: body curled up, weight loss,unresponsive, angular, eat less cold, and cold limbs, Mao Song,vertical hair, lose luster and hair removal.In the Morris water mazetask.2.the escape latency made by the model group was remarkablylonger than that of the Young Group (P<0.01); the escape latencyof the Young Group is shorter than EA Group and EAC group (P<0.05); there was differences between EA Group and EAC group (P<0.05).3.the firt acrossing time in model rats was longer than theyoung group rats, and it was shorten by the treatment of EA, therewere significant difference between them（P＜0.01）,the spaningtimes also increased through the treatment of EA, there weresignificant difference between them（P＜0.01）.4.The record after HFS indicated that the incidence of LTPinduction of rats and PS amplitude in the model group wassignificantly lower than that of the Young Group, EA Group and EACgroup (P<0.01); In comparison with the Young Group, there was nosignificant difference between Young Group， EA Group and EAC group(P>0.05); there was no significant difference between EA Group andEAC group (P>0.05).5.In comparison with the Young Group, the length of active zonesand the thickness of PSD are all decreased, the synaptic cleft widthenlarged significantly the model group（P＜0.01）; there was nodifferences between EA Group and EAC group（P>0.05）；In comparisonwith the model Group, the thickness of PSD are increased, and thesynaptic cleft width decreased remarkably（P＜0.01）; there was nodifferences between EA Group and EAC group（P>0.05）；the lengthof active zones enlarged in the EA Group（P＜0.01）; there was nodifferences in the EAC group（P>0.05）；In comparison with the YoungGroup, the synaptic interface curvature with no significantdifference in model Group, EA Group and EAC group（P>0.05）.6.compared with Young Group, the content of BDNF declinedremarkably in model Group（P＜0.05）; compared with model Group,the content of BDNF in EA Group increased（P＜0.05）；there was nodifferences in the EAC group（P>0.05）; in comparison with the EA Group, the content of BDNF in EA Group decreased significantly（P＜0.05）.7.compared with Young Group, the expression of PKC mRNAdeclined remarkably in model Group（P＜0.01）; in comparison withthe model Group, the expression of PKCmRNA raised remarkably in EAGroup and EAC group（P<0.01）；there was no differences between EAGroup and EAC group（P>0.05）； compared with Young Group, therewas no differences between EA Group and EAC group（P>0.05）.8.compared with Young Group, the expression of NMDAR declinedremarkably in model Group（P＜0.01）（P<0.01）；the expression ofNMDAR decreased in EAC group（P<0.05）；compared with EAC group，the expression of NMDAR raised remarkably in EA Group（P<0.05）；there was no differences between EA Group and Young Group（P>0.05）.ConclusionThe above results suggest that the Shuanggu Yitong acupuncturemethod can improve the spatial learning and memory in aging modelrats with Yang-deficiency;This efficency was obtained through thetreatment increased the incidence of LTP induction at thehippocampal dentate gyrus in the aging model rats; and the methodcan improve the hippocampal synaptic structural parameters; andadjust and regulate content of BDNF and the PKC mRNA expression，and the NMDA receptor expression in aging model rats withYang-deficiency.Therefor, the Shuanggu Yitong acupuncture methodcan improve the ability of learning and study in rats withYang-Defficiency, the mEAChanism was connEACted with ShuangguYitong acupuncture method can protEACt the instruct of Hippocampustissue and regulate the content of BDNF and PKCmRNA and NMDARaffEACted the Long-Term-Potentiation, but the more sEACret about them should be explore out by more researching in the future. |
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