The Effect And Mechanism Of Shenqiyixin Recipe On Energy Metabolism Of H9C2Cardiomyocytes

Objective:To observe the protective effect of Shenqiyixin Recipe on H9C2cells induced by norepinephrine, and to investigate the Shenqiyixin Recipe’s adjustment effect on energy metabolism of H9C2cells, explore the therapeutic mechanism of Shenqiyixin Recipe on heart failure.Methods:H9C2rat cardiomyocytes were divided into normal control group, model group, Trimetazidine group and Shenqiyixin group. The last three groups were added with normal rat serum10μL, serum with10μL Trimetazidine, serum with10μL Shenqiyixin Recipe and each group added norepinephrine (final concentration was2umol/L), indicators were tested at the24h,48h. cardiomyocytes survival was detected by MTT,Metabolic rate of fatty acids and glucose were detected by blinking fluorescence,and HPLC were unutilized for detecting the ATP generation from damaged myocardial cell. immunoblotting were unutilized for detecting the expression of F1-ATPase、PPARaResults:compared with model group, Shenqiyixin Recipe group and Trimetazidine group could significantly increase OD value(P<0.01), the value of Shenqiyixin Recipe group was significantly higher than Trimetazidine group (P<0.01); compared with model group, the fatty metabolic rate of Shenqiyixin Recipe group and Trimetazidine group were significantly lower (P<0.01), and fatty metabolic rate of Shenqiyixin Recipe group was significantly lower than Trimetazidine group(P<0.01); the glucose metabolic rate of Shenqiyixin Recipe group and Trimetazidine group were significantly higher (P<0.01), and glucose metabolic rate of Shenqiyixin Recipe group was significantly higher than Trimetazidine group(P<0.01);compared with model group and Trimetazidine group. The expression of F1-ATPase in Shenqiyixin Recipe group was significantly higher and the expression of PPARa in Shenqiyixin Recipe group is significantly lower. Conclusion:1. Shenqiyixin Recipe could effectively protect the H9C2cells induced by norepinephrine.2. Shenqiyixin Recipe could effectively adjust the energy metabolism of damaged cardiomyocytes. by inhibiting the expression of PPARa which down regulating the fatty acid metabolism, the damaged cardiomyocytes could generate ATP efficiently by the glucose metabolic way to maintain cardiomyocytes metabolism.3. Shenqiyixin Recipe could effectively protect the cardiomyocytes’MMP and adjust the energy metabolism of damaged cardiomyocytes. It may be the mechanism for Shenqiyixin Recipe treating heart failure...