Baicalin Inhibits Breast Cancer Through MiR-126Expression

Objective Screen differential miRNA which is regulated by the baicalin, from the perspective of miRNA,to explore the approaches and mechanism of the inhibitory effect of Baicalin on breast cancer.Methods In vitro experiments effect on breast cancer cell MCF-7、 MDA-MB-231by different concentrations of baicalin, Immunohistochemical analysis showed that the lowest effective drug concentration, by using miRNA chip,screening differentially miRNA by drug regulation. qRT-PCR for upregulated miRNA microarray results validate, and then detect cell survival by MTT assay, detection of cell invasion and metastasis by transwell experiment, detection of apoptosis-related protein expression by western blot and flow cytometry to detect apoptosis.clinical trials testing cancer tissue and normal tissue cytokine’s immunohistochemistry difference and qRT-PCR to detect differences in miRNA expression.ResultsIn vitro experiments1) After baicalin effect on breast cancer cell, TGF-β(transform growth factorp), VEGF(vascular endothelial growth factors), immunohistochemical score decreased, the best concentration was50μmol/l;2) baicalin effect on breast cancer cell, microarray screens miR-126expression increased most;3) baicalin and miR-126effect on breast cancer cell, the rate of cell growth and invasiveness was significantly lower;4) baicalin and miR-126effect on breast cancer cell,the expression of Caspase-3(Cysteinyl aspartate-specific proteinase-3), Caspase-9(Cysteinyl aspartate-specific proteinase-9), Bax (Bcl-2Associated X Protein) and P53increased and the expression of Bcl-2(B cell lymphoma2) decrease, at the same time P-38(mitogen-activated protein kinase) activation also increased;5) baicalin and miR-126effect on breast cancer cell, apoptosis of breast cancer cells increased;Clinical trials1) compared with normal mammary gland tissue, immunohisto-chemical score of TGF-β, VEGF significantly increased in breast tissue;2) The relative expression of miR-126in breast cancer tissue was significantly lower than normal breast tissue;Conclusion1) Expression of miR-126in breast cancer cells decrease;2) Baicalin upregulates tumor suppressor miRNA genes such as miR-126;3) By upregulating the expression of miR-126, baicalin inhibits the proliferation and invasion, probably inhibit metastasis of breast cancer cells.