Effect Of MiRNA-630 On The Proliferation Of Triple Negative Breast Cancer Cells With High BRCA-1 Expression

Background and Objective In recent years,the research and application of microRNAs(miRNAs)involving various diseases have attracted more and more attention in the field of biology.It plays a post-transcriptional regulation role in all eukaryotes,and can regulate gene expression of various physiological processes and intracellular singal transduction.Its abnormal expression has has cancer-causing or tumor-suppressive functions that are involved in the occurrence,invasion and metastasis of cancer.Among them,miRNA-630 has been shown to be involved in the occurrence and progression of a variety of tumors,including breast cancer,providing an experimental basis for the treatment of breast cancer.For tumor subtypes that lack effective,specific therapeutic targets,such as triple-negative breast cancer(TNBC),which is highly expressed by breast cancer susceptibility gene(BRCA-1),miRNA-630 may also be a new treatment.The use of miRNA-630 as an adjunct to treatment seems to have some promise.The purpose of this study was to investigate the effect of miRNA-630 on the proliferation of TNBC cells with high expression of BRCA-1,and finally provide new ideas for the prevention,diagnosis and treatment of TNBC.Methods miRNA-630 mimic,BRCA-1,miRNA-630mimic+BRCA-1,NC,miRNA-630 inhibitor,miRNA-630inhibitor+BRCA-1 were transfected into TNBC cell line(MDA-MB-231)cultured in vitro.qRT-PCR was used to detect the expression level of miRNA-630 in NC,miRNA-630 mimic and miRNA-630 inhibitor.The proliferation of MDA-MB-231 cells was detected by MTT,and the expression of P21 and P53 in MDA-MB-231 cells was detected by Western blot.Results 1.PCR results showed that miRNA-630 mimic increased miRNA-630 expression level compared with empty plasmid group(p<0.05),while miRNA-630 inhibitor significantly reduced miRNA-630 expression level(p<0.05).2.The results of MTT and Western blotshowed that(1)compared with the empty plasmid group,the proliferation of MDA-MB-231 cells decreased(p<0.05)and the expression levels of P21 and P53 increased after transfection of BRCA-1.<0.05),the difference was statistically significant.(2)Compared with the empty plasmid group,the proliferation of MDA-MB-231 cells was decreased(p<0.05),and the expression levels of P21 and P53 were increased(p<0.05).The difference was statistically significant.(3)Compared with MDA-MB-231 cells transfected with BRCA-1,the proliferation of MDA-MB-231 cells was decreased(p<0.05),P21 and P53 expression levels were transfected with miRNA-630mimic+BRCA-1.Increased(p < 0.05),the difference was statistically significant.(4)Compared with the empty plasmid group,after transfection of miRNA-630 inhibitor,the proliferation of MDA-MB-231 cells increased(p<0.05),and the expression levels of P21 and P53 decreased(p<0.05).The difference was statistically significant.(5)Compared with MDA-MB-231 cells transfected with BRCA-1,the transgenic miRNA-630inhibitor+BRCA-1 increased the proliferation of MDA-MB-231 cells(p<0.05)and decreased the expression of P21 and P53.(p<0.05),the difference was statistically significant.Conclusion miRNA-630 is a tumor suppressor in the BRC-1 high-expressing TNBC cell line and has a negative regulatory effect,which may become a biotherapeutic target of TCNBC with high expression of BRCA-1 in the future,which is the prevention,diagnosis,and prevention of TNBC.Provide scientific basis for treatment.