The Mechanism Of CAMP/PKA Signaling Pathway In Rats With Vascular Dementia And Intervention Studies Of Yifei Xuanfei Jiangzhuo Fang

Vascular dementia(VD),resulting from brain loss caused by varieties of cerebrovascular diseases, is an acquired intelligent damage syndrome and cognitive function defect.Cerebrovascular diseases lead to atherosclerosis, and ischemia or ischemia-reperfusion injury will appear, which results in dementia.Studies have shown that as one of the important signal pathways in the formation long-term potentiation (LTP) process,cAMP-PKA-CREB signaling pathway plays an important role in regulating the process of learning and memory formation,which can promote the survival of nerve cells, regeneration and differentiation.On the basis of blood circulation resonance theory and Qi cycle theory,this text presents the theory Treating VascularDementia from the physiological function of the lung and the and effective prescription Yifei Xuanfei Jiangzhuo fang.The aim of the present study was to investigate the effect of cAMP-PKA-CREB signaling pathway on learning and memory activities in a rat model of vascular dementia (VD) with a variety of experimental methods.First Part:Theory researchFirstly,recall ancient physicians knowledge about VD and discuss the research status of Chinese medicine treatment of vascular dementia.Secondly,we think that the pathogenesis of vascular dementia is that the carotid artery or stenosis of cerebral arteries,hardening,decreased flexibility,which causes higher vibration frequency,makes the blood can not fully enter the brain,lack of blood supply to the brain,affects the brain’s normal function,and then causes the memory loss even dementia.On the basis of Blood circulation resonance theory and Qi cycletheory,This text presents the theory Treating Vascular Dementia from the physiological function of the lung and the and effective prescription Yifei Xuanfei Jiangzhuo fang.Second Part:Empirical researchPurpose:Establish the vascular dementia rat model and observe the expression changes of protein associated with cAMP-PKA-CREB signaling pathway after the intervention of Yifei Xuanfei Jiangzhuo fang in VD rats.Method:(1) Selecting the big mice of Wistar,sets up the VD model,all the VD rats were randomly divided into six groups:Model group,the high doses group,the Moderate doses group,the lower doses group,the amount of pharmaceutical group,comparison group huperzine,comparison group pisacetam.All the rate were tested by Morris water maze after30days continuous perfusion;(2) Morris water maze test:Test the behavioral changes in VD rats by Morris water mazeverify the success and impact modeling Yifei Xuanfei Jiangzhuo VD rats learned behavior and observe the effect of Yifei Xuanfei Jiangzhuo on the behavior of VD rats,(3) Pathological structures:Observe the morphological changes in rat hippocampus and the effect of Yifei Xuanfei Jiangzhuo fang on the behavior of VD rats using the Light microscopy,electron microscopy,TUNEL method.(4) Detection of the proteins related to the cAMP-PKA-CREB pathway:Observe the expression changes of the protein cAMP,PKA,p-CREB,associated with cAMP-PKA-CREB signaling pathway after the intervention of Yifei Xuanfei Jiangzhuo fang in VD rats.Result:(1)Morris water maze test results:Compared with the model group,the escape latency of Yifei Xuanfei Jiangzhuo group were significantly shortened and the number across platforms after the removal of the platform significantly increas-ed;(2)pathological structures results:Compared with the model group,the number of hippocampal neuronal apoptosis of Yifei Xuanfei Jiangzhuo fang group were significantly reduced; (3)ELISA and Western blot results:Compared with the model group,the expression of the protein cAMP, PKA and p-CREB of Yifei Xuanfei Jiangzhuo fang group is significantly increased.Conclusion:(1) Results of all the tests show that:Yifei Xuanfei Jiangzhuo could Improve memory of vascular dementia rats significantly,inhibit apoptosis in rat hippocampal neurons,protect neurons;increase the expression of the proteins related to cAMP-PKA-CREB signaling pathway. Based on the research above,we sugges:Yifei Xuanfei Jiangzhuofang may function by raising cAMP,PKA,p-CREB expression in hippocampal neurons,activating the cAMP-PKA-CREB signaling pathway,promoting the formation of LTP,protecting the neurons,further improving learning and memory ability of VD rats.(2) Results of all the tests show that:Yifei Xuanfei Jiangzhuo fang has a better therapeutic effect on vascular dementia rats, suggest that the theory Treating vascular Dementia from the physiological function of the lung is feasible,and worth further exploring.