A Study On The Role In Repairment Of Diabetic Retinal Microvascular Injury Treated With The Collateral Theory

Objective: For diabetic retinopathy (DR) characteristics of consumptive diseasepathogenesis of collaterals, by collateral disease as the breakthrough point to evaluate thethe role in repairment of diabetic retinal microvascular injury treated with the collateralneedling and drug。 Methods: Using rats as experimental subjects， by injection ofstreptozocin (STZ) into DR model，after treatment with Dahuang Zhechong pillsintragastric administration and collateral puncture，in observation of the followingindicators:1.Rat body weight、blood glucose、glycosylated haemoglobin、plasmafibrinogen and the incidence of cataracts.2. By fundus fluorescein angiography (FFA), toobserve the changes of retinal vessel diameter of DR rats, fluorescein leakage area andshape.3.By paraffin section of retinal tissue combined with HE staining method,to observethe change of every layer of retina tissue structure under light microscope.4.By paraffinsection of retinal tissue combined with immunohistochemical method,to observe theexpression of retinal nuclear factor-κb (nf-κb)。5. Through the retinal digest preparationscombined with PAS staining, to observe retinal microvascular changes inmorphology.6.Through the retinal digest preparations combined with immunofluorescencetechnique, to observe the expression of vascular cell adhesion molecule-1(VCAM-1) inretinal vascular wall.Results:1.Collaterals therapy can significantly change the symptomsof“three more and one less”in DR rats, reduce the effect of high glucose on rat bodyweight,and reduce blood fibrinogen content.2.FFA showed that collaterals therapy canreduce the generation of DR rat retinal neovascularization，and can reduce vascularleakage.3. Morphological observation of retinal tissue biopsies showed that collateralstherapy can significantly reduce the degree of retinal edema, reducing neuronal damage,inhibit tumor angiogenesis.4.Immunohistochemistry showed collaterals treatmentdecreased expression of nf-κB in retina.5.Retinal digest preparations combined with PAS and immunofluorescence showed collaterals therapy can improve the microvascularmorphology, and reduce the expression of inflammatory factor VCAM-1in the vascularwall.Conclusion: Collateral treatment can effectively alleviate the hyperglycemia inducedby retinal microvascular injury, improve diabetic retinal structure and function of bloodvessels, change the microvessel wall permeability, inhibit or eliminate induced hemorrhageand exudation, thus realizing the retinal protection. Its mechanism may be through theregulation of fibrinogen in plasma content, reducing the blood viscosity, increase the flowand oxygen partial retinal blood supply, inhibit the expression of retinal nf-κB activationand downstream of the VCAM-1and other inflammatory factors. The above researchresults in the rich of traditional Chinese collaterals theory,and at the same time providesnew ideas and methods for the prevention and treatment of DR.