| Objective:We chose senescence-accelerated mouse prone8(SAMP8) as Alzheimer’s Disease model and tested the hippocampal long-term potentiation (LTP) of these mice after a fixed period of acupuncture treatment.LTP result is to comfirm the electric physiological effects and active influences on learning and memory ability.At the same time, the changes of ultra microstructure and calmodulin in hippocampal nerve cells can reveal the mechanism of AD nerve recovery gained by acupuncture treatment.Subjects and Experimental Paradigm:1.Defining the specific locations of electrode needle for different kinds of mice;2.The influences on LTP of normal mice under the different course of acupuncture treatment is observed to estimate our experimental conditions.The Balb/c mice were chosen, weighed20to30grams.Acupuncture treatment included two points "Baihui acupoint (GV20)" and "Yongquan acupoint(KL1)" and puncturing once per day.The subjects differed by different courses.They are2-week with no measures,2-week with holding,2-week with acupuncture,4-week with no measures,4-week with holding, and4-week with acupuncture.The mice was anaesthetized after treatment and places on stereotaxic apparatus to test LTP;3. SAMP8mice aged9months were chosen as AD animal model.They were randomly divided into two groups, control group and acupuncture group.The same aged male homologous SAMR1mice were chosen as the normal group.The LTP test was applied after4weeks treatment.Transmission electron microscope combining with stereological method was used to observe and measure the synaptic ultrastructure in hippocampal neurons of CA1subregion.Western blot were used for the phosphorylation CaMK Ⅱ. Results:1.We fixed the specific locations of electrode needle for four kinds of mice;2.The analysis of LTP results in Blab/c mice demonstrate that (1)There exists no difference in PS and Latency between2-week groups;(2) there is a significant difference between4-week groups in terms of PS growth-rate(P<0.05);(3) There is a significant difference between4-week normal group and4-week control group in PS(P<0.05);(4)There is a significant difference between4-week control group and4-week acupuncture group in PS(P<0.05);(5) There exists no difference in Latency among three4-week groups;3.The comparison of LTP between SAMP8and SAMR1groups shows that:There are significant differences among three groups both in PS and Latency (P<0.05).The P8acupuncture group are better than R1normal group both in PS increases and Latency declines (P<0.05). The result of Latency declines of SAMP8acupuncture group is significantly higher than SAMP8control group (P<0.05). P8control group has no significant difference compared with R1normal group in PS and Latency;4. In comparison to the control group, the thickness of PSD (P<0.05) and the curvature of synaptic interface (P<0.05) decreased, the width of synaptic space increased (P<0.05) in hippocampal neurons of CA1area in the model group, while the thickness of PSD(P<0.05) and the curvature of synaptic interface(P<0.05) was increased, the width of synaptic space decreased (P<0.05)5.There were no significant differences in the total quantity of phosphorylated CaMK Ⅱ in hippocampus among the three groups (P>0.05)Conclusion:Our data reveal that 1. There might be a correlation between LTP of normal mice and the bound under the condition of acupuncture.But acupuncture treatment has rivalry effect on deficiency of memory and study ability.2. The acupuncture treatment can improve the memory and study ability of AD mice by changes related with synaptic plasticity.3. The influences from acupuncture might attribute to the improvements in nerve structures which also means the more active synaptic transmission, which implied the mechanism that acupuncture therapy can contribute to nerve recovery in AD diseases. |
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