Related Factors Intraepithelial Cervical Colposcopic Biopsy Pathology Of Two

Background and ObjectCervical intraepithelial neoplasia (CIN) is a series of progressive precancerous changes caused by the infection of human papillomavirus (HPV). They are graded as CIN1, CIN2and CIN3in order of increasing severity. CIN2is sorted of high graded lesions like CIN3. A diagnosis of CIN2usually triggers surgical treatment. But the biological features of CIN2are far different from those of CIN3. Only a very small percent of CIN2will progress into invasive cancer and a large part of CIN2may regress spontaneously. Many patients with colposcopical biopsy-confirmed CIN2may receive a diagnosis of CIN1or less after surgery. To avoid the overtreatment, these patients should be triaged in a proper way. P53, Bcl-2and survivin are three critical proteins in many human malignancies. It’s reported that the expressions of these proteins are all related to the grade of lesions of the cervical. The object of this research is:1) to confirm the correlations between the expressions of p53, Bcl-2, survivin in biopsy specimens and the pathological diagnoses based on the surgical tissues2) to confirm the correlations between the ages, the cytological interpretations, the HPV testing results of the patients and the pathological diagnoses.MethodsA database was created using information about patients who received surgical treatment for biopsy confirmed-diagnoses of CIN2in Peking Union Medical College Hospital between September2003and January2011. The biopsy tissues were immunohistochemically stained for p53, Bcl-2and survivin. The analysis of the stain results was performed by the Image Pro Plus software. And SPSS17.0was used for statistical analysis.ResultsThe cytological interpretations were weakly correlated with the postoperative diagnoses based on surgical samples (r=0.265, P=0.048<0.05). The expressions of p53and Bcl-2in the biopsy tissues were both positively correlated with the postoperative diagnoses (r=0.300, P=0.025<0.05; r=0.308, P=0.021<0.05, respectively) but not the expression of survivin. Neither the HPV testing results nor the ages of the patients had correlation with the final diagnoses.ConclusionThe expressions of p53and Bcl-2in biopsy spicemens of CIN2were positively correlated to the diagnoses based on surgical tissues. They might be candidates for the triage of patients with biopsy-confirmed CIN2.