| Objective:To explore the influence of Baihu Erdi decoction on treating T2DM insulin resistance in rats blood glucose, blood lipid, inflammatory cytokines and intervention of skeletal muscle PI3K signaling pathways, explore the inflammatory cytokine of T2DM insulin resistance rats factor the formulas and the influence of the PI3K signaling pathway and how did these produce.Method:T2DM insulin resistance rats were established by feeding with high-sucrose-fat diet for 8 weeks followed with streptozotocin (STZ) (30mg/kg, i.p). Rats were rando mly divided into normal and diabetic group. After modeling, diabetic group were random ly divided into untreated diabetic group, rosiglitazone group, and two Chinese medicine g roups with different doses of Baihu Erdi decoction. After 6 weeks intervention, indicators as fasting blood glucose (FBG), fasting insulin (FINS), insulin sensitivity index(ISI), h omeostasis model assessment-insulin resistance (HOMA-IR), serum total cholesterol (TC), triglyceride (TG), free fatty acid (FFA), high density lipoprotein cholesterol (HDL-C) an dlow density lipoprotein cholesterol (LDL-C),tumor necrosis factor a (TNF-a) interleukin-6 (IL-6)were measured before and after intervention. Liver, skeletal muscle and fat pathol ogical changes of five groups were observed through a microscope.Insulin receptor substr ate-1 (IRS-1), anti-phospho-IRS-1 (p-IRS-1), the eighty-five protein of phosphatidyl inosit ol 3-kinase, AKT (B protein kinase) and p-AKT were detected from skeletal muscle org anization by using western blot.Results:1. T2DM insulin resistance rats were established by feeding with high-sucrose-fat diet followed with streptozotocin (STZ)compared with normal group, the FBG、FINS、HOMA-IR、 ISI increased significantly, the difference is statistically significant (P<0.05)2. After drug intervention, Baihu Erdi decoction high dose group of SD rats liver tissue in fatty infiltration reduced,adipose tissue atrophied,FBG, FIMS, HOMA-IR, the ISI, TC, TG decreased, is superior to low dose group of SD rats,compared with model group, the differences were statistically significant (P<0.05).ISI showed no significant differences between each test groups and model group (P>0.05).3. After drug intervention, Baihu Erdi decoction high dose group of SD rats and low dose group of SD rats in FFA, TNF-a, IL-6 were declined significantly compared with model group, the difference is statistically significant (P<0.05).And the effects of Baihu Erdi decoction high dose group of SD rats is better than Baihu Erdi decoction low dose group of SD rats.4. After drug intervention, Baihu Erdi decoction high dose group of SD rat the IRS-1 protein expression quantity in skeletal muscle tissue increased compared with model group, the difference is statistically significant.And the effects of Baihu Erdi decoction high dose group of SD rats is better than Baihu Erdi decoction low dose group of SD rats.5. After drug intervention, Baihu Erdi decoction high dose group of SD rat skeletal muscle, INSR-beta, PI3Kp85, AKT expression quantity compared with the model group increased significantly, the difference is statistically significant.Conclusion:1.Baihu erdi decoction supplementation could improve metabolic disturbance and reduce insulin resistance of type 2 diabetic rats with insulin resistance. It is related to reducing lipotoxicity and enhancing insulin resistance of target tissues.2.Baihu erdi decoction can reduce T2DM in different degree of insulin resistance in rats serum FFA, TNF alpha, IL-6 level, so as to reduce inflammation reaction.Its possible mechanism is Baihu erdi decoction of hypoglycemic lipid-lowering, relieve insulin resistance can reduce inflammatory reaction.3.Baihu erdi decoction can increase amount of T2DM insulin resistance rats IRS-1 expression and activation of PI3K signal transduction pathway, promote the metabolism of glucose, improve insulin resistance.4.Baihu erdi decoction can improve the T2DM insulin resistance induced by STZ rats inflammation, relieve insulin resistance. Its possible mechanism is by inflammatory signaling pathways, reduce inflammation, the expression of PI3K protein increase muscle tissue, thereby weakening peripheral insulin resistance. |
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