Muscle Inositol, D-chiral Inositol And The Combination Of The Two On The Insulin Resistance Of HepG2 Insulin Resistance Cells To Improve The Role

Aims: To compare the effect of myo- inositol( MI),D-chiro- inositol( DCI) and their synergist on improving glucose consumption of insulin-resistant Hep G2 cel(Hep G2-IR), and explore the possible mechanism.Methods: 1. Effect of glucose(0、25、35、45、55、65mmol/L) on the activity of Hep G2 cells, and among 0、0.25、0.5、1、2、4、8、16、32mmol/L, MI,DCI, and their synergist on the activity of Hep G2 cells was detected by CCK-8 assay to determine their dose scope in the experiment. 2. The Hep G2-IR cell model was established by 55mmol/L glucose for 24 h and 1nmol/L insulin stimulation for 15-25 min. The successful authentication of the model was determined by glucose oxidase method(GOD-POD). 3. The glucose consumption of the Hep G2-IR cell treated with MI, DCI and their synergistic treatment groups( 0.125 、 0.25 、 0.5mmol/L) were determined by GOD-POD. The expression of PI3 K, Akt and phosphorylation of Akt(p Akt) protein was determined by western blotting.Results: 1. Compared with the blank control group, glucose(35~55mmol/L) had no valid influence on the Hep G2 cells, and glucose(65mmol/L) had significantly inhibitory effect on the Hep G2 cells(P< 0.05); MI, DCI(0~0.5 mmol/L) activity had no effect on the Hep G2 cells, MI, DCI(1 ~ 32 mmol/L) had significantly inhibitory effect on Hep G2 cell activity(P < 0.01), and with the increase of the concentration, the cell activity had been gradually reduced; their synergistic treatment groups(0~1 mmol/L) had no valid influence on the Hep G2 cells, their synergistic treatment groups(2 ~ 32 mmol/L) had significantly inhibitory effect on Hep G2 cell activity(P < 0.01), and with the increase of the concentration, the cell activity had been gradually reduced. 2. The GOD-POD analysis found that compared with normal control group, glucose consumption of the Hep G2 cells, which was induced by 55mmol/L glucose for 24 h and 1nmol/L insulin stimulation for 15-25 min was decreased significantly, showing the successful authentication of Hep G2-IR cells model. 3. The GOD-POD analysis found that compared with model control group, glucose consumption was significantly higher in the MI, DCI and their synergistic treatment groups(P <0.05), with the increase of intervention concentration, the glucose consumption of each group had been gradually increased, and glucose consumption in synergistic treatment groups was most significantly in the same concentraton(P < 0.05). 4. Western blotting analysis found that compared with model control group, expression of PI3 K, p Akt / Akt was significantly higher in the MI, DCI and their synergist ic treatment groups(P<0.05), with the increase of intervention concentration, expression of PI3 K, p Akt / Akt in each group had been gradually increased, and expression of PI3 K, p Akt / Akt in synergistic treatment groups was most significantly in the same concentraton(P < 0.05).Conclusions: MI, DCI and their synergistic team could improve expression of PI3 K and promote phosphorylation of Akt on Hep G2-IR cell, which further increased glucose consumption of Hep G2-IR cell, so as to improve IR, and the effect of their synergistic team was the most significantly.