The Effects Of Expression Level Of PTP1B And PI3K In Rat Skeletal Muscle During Intermittent Hypoxia On Insulin Resistance

Objective:Through testing the protein expression of protein tyrosine phosphatase-1B(PTP1B) and phosphatidylinositide 3 kinase(PI3K) of intermittent hypoxia in rat skeletal muscle cells,to investigate the changes of intermittent hypoxia in the insulin pathway and reflect the possible mechanism of insulin resistance(IR). Method:Selected 24 healthy male SD rats were randomly divided into intermittent air control group(NC group), intermittent hypoxia 4 weeks group(IH4 group) and intermittent hypoxic 8 weeks(IH8 group) with 8 rats in each group. The intermittent hypoxia 4 weeks group and 8 week group rats placed intermittent hypoxia cabin, exposure to intermittent hypoxia environment 8 hours per day, the control group was given intermittent compressed air. NC group and IH8 group in 8 weeks, IH4 group in 4 weeks after fasting for 12 hours, then the rats were measured the fasting blood glucose(FBG), fasting insulin(FINS), using the homeostasis model assessment(HOMA) insulin resistance index(HOMA-IR); To observe the morphological changes of rat skeletal muscle cells with hematoxylin eosin(HE) staining and observe the injury degree of intermittent hypoxia on rat skeletal muscle cells, using immunohistochemical method to detect the expression of PTP1 B and PI3 K protein in skeletal muscle cells, taking the average gray value of the protein expression, and giving a statistical analysis of data. Results:Compared with the NC group,the experimental groups elevated FBG,FINS,HOMA-IR, the difference was statistically significant; Compared with the NC group, the expression of PTP1 B protein was increased in IH4 and IH8 groups, especially in IH8 group, F values were 596.91,P<0.05;but the PI3 K was decreased, F values were 128.47,P<0.05.Pearson correlation analysis showed that HOMA-IR and the expression of PTP1 B protein was negatively correlated and was positively correlated with PI3 K protein.HE staining showed that the skeletal muscle cells in NC group myofilament closely;but in the intermittent hypoxia groups, rat skeletal muscle cells were thin, fuzzy cell morphology,larger gap in bundles and the transverse fracture pattern. Conclusion:Intermittent hypoxia can lead to the increase of fasting blood glucose and fasting insulin, insulin resistance, with intermittent hypoxia exposure time prolonged, increased insulin resistance. Intermittent hypoxia of PTP1 B and PI3 K expression in rat skeletal muscle cells might be involved in the occurrence of insulin resistance induced by intermittent hypoxia.