| Background:Rheumatoid Arthritis (RA) is a kind of symmetry Multi-joint Synovitis, Synovial abnormal increase into chronic or systemic utoimmune diseases. The appearance of this symptom is mostly seen in T lymph cells, moisture Monocytes, the increase of B Lymph cells, Plasma cells and Collagenase inside the Synovial fluid; but specific mechanism have not yet been fully discovered. "Bitongling" was developed by the late Chinese medicine doctor, Dr. Wang, on the basis of his experience. The clinical trials related to the treatment of Rheumatoid Arthritis have obvious efficiency.Method:1. The main medicine research is to observe the effect of Bitongling’s to the T cells mediated cellular immunity model -Adjuvant Arthritis effect.2. By using analgesic experiments with hot plate and writhing methods to examine the analgesic effect of the rat taking Bitongling.3. The result of observing the effect of microcirculation on rats applied by Bitongling after the experiment of microcirculation on mesenteric of rats.4. Examine Bitongling’s effect to the rat’s pathological model (Adjuvant arthritis) in the splenocytes to induce NO, MMP, NO blood serum and MMP released by T lymphocytic.5. Oral administration of Bitongling Granule significantly inhibited the SRBC-DTH, the ear edema induced by Xylene, the Paw swelling induced by carrageenan, and the leukocyte migration in abdominal cavity of rats.6. On the level of the cells, to examine effect of Bitongling on the activation proliferation of T lymph cells and the movement of inflammation.7. Observe the effect of Bitongling on PCI-CS, which the rat’s splenocytes produces MMP-2, MMP-9 and NO outside of the body.Results:1. Through analgesic experiments by using hot plate and writhing methods, it can be seen that Bitongling can increase the rat’s pain threshold. Reducing the rat’s writhing that was stimulated by the acetic acid, showing that Bitongling does have pain reducing effects.2. By Oral administration of Bitongling, the level of 27.5g/kg,13.75g/kg, and 6.87g/kg could increase the number of opened cross points of capillary for the microcirculation on mesenteric of rats, and significantly increase velocity blood flowing in the capillary by improving the status of blood flow.3. Bitongling can obviously reduce the rat’s NO serum level of Adjuvant arthritis, suppressing the rat’s splenocytes to secreting NO, reduce arthritis secreting MMP-2 and MMP-9.4. The research showed that the effect of the Bitongling granule on delayed type hypersensitivity (DTH) induced the sheep’s red blood cells (SRBC), ear edema induced by xylene, paw swelling induced by carrageenan, and leukocyte migration in the abdominal cavity of rats.5. Bitongling is effective towards the activation proliferation of T lymph cells and the movement of inflammation. Compared with Ciclosporin, the results show that Bitongling are effective to the survival of healthy lymphocytes.6. In extracellular medium, Bitongling can inhibit PCI from attacking for up to 6 hours, the activeness of MMP-2, MMP-9 created by splean lymph cells with the stimulation of Con A, and the production of NO.Conclusion:Oral administration of Bitongling can inhibit the reaction of delayed hypersensitivity by mainly inhibiting the activity of lymphocytes infiltrating into the inflammation site, including the adhesion of lymphocytes to the extracellular medium, the release of MMp and NO to its subsequent migration process. Also, the cellular immune-pathological model can be suppressed by inhibiting the activation of lymphocyte function. |
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