The Relationship Of Kidney Deficiency And Phlegm Stasis With AD, And The Research For The Therapy Of Bushen-Huatan-Kaiqiao Method To AD

Objective: Vitality Consolidation Treatment has a very important impact on the formation and development of TCM treatment system. In this article, we had reviewed systematically the cognitions of Ancient doctors in senile dementia, and thinking that the pathogenesis of senile dementia is insufficiency of the kidney and phlegm and stagnant blood clogging aperture,which is the syndrome of deficiency in origin and excess in superficiality. To explorer the etiological factor and effect of replenishing kidney-essence and remove phlegm therapy on treating Alzheimer’s disease(AD) in theory. APP/PS1 double transgenic mice is used as AD model to observe the effects of replenishing kidney-essence and removing phlegm therapy on learning memory, the activity of cholinergic system, pathological observation and the ubiquitin-protea-some passway in the hippocampus and cerebral cortex to provide the the theoretical basis and experimental evidence to further theoretical research and clinical research of Vitality Consolidation Treatment.Method: 1.Theoretical discussion. Collate the ancient and modern literature about the replenishing kidney-essence and remove phlegm therapy on treating Alzheimer’s disease(AD) in theory, summarize the basic concepts, theoretical connotation, generation, distribution, cognitive function and other contents. Clarify the theoretical basis of the relationship among the replenishing kidney-essence and remove phlegm therapy on treating Alzheimer’s disease(AD) in theory. 2. Experimental study. APP/PS1 double transgenic mice is used as AD model, the ubiquitin-protea-some passway used as the target, to study the effect of the Yizhiwendan Granules to AD. Morris water maze and diving platform as the ability of learning and memory. Western Blot to detect the main protein of ubiquitin. Real Time-PCR to detect the main m RNA expression of ubiquitin IHC method to detect the protein of ubiquitin in mice’s hippocampus CA1.Result 1. Morris water maze test showed that compared with the control group, Mice in model group’s platform latency, the first time reach for the original platform and total swimming distance increased significantly(P<0.01), through platform number and the target quadrant time reduced significantly(P<0.01). Compared with the model group, each group’s platform latency and total swimming distance reduced(P<0.05), the target quadrant time increased(P<0.05). The Phlegm group and The kidney group group’s through platform number increased(P< 0.05), and presents a certain concentration-response relationship between two groups. Compared with Donepezil group, other group presents no certain concentration-response relationship between two groups(P>0.05). 2. Diving platform test showed that compared with the control group, Mice in model group’s wrong number increased significantly(P<0.01), latency period reduced significantly(P<0.01). Compared with the model group, Donepezil group and other groups wrong number reduced(P<0.05); Donepezil group and Changpuyizhiwan group’s latency period increased(P<0.05). 3. The result of light microscope have approved that the volume of neuron of hippocampus decreased and denaturated in model group and replenishing kidney-essence,removing phlegm therapy can recovery those pathological change. 4. IHC showed that the control group mice’s hippocampal CA1 region’s cells were complete, in alignment, normomorph, cell membrane and cytoplasm with deep brown granules. The model group’s cells were disordered arranged and shallow dyed. Compared with the model group, each group’s cells were in alignment light stained. 5. Western Blot showed that compared with the control group, mice’s hippocampus in model group’s ubiquitin relative transcript level increased(P<0.01). Compared with the model group, Changpuyizhi Pilule and Yizhiwendan Granules group’s ubiquitin level reduced(P<0.01); Donepezil group and The kidney group ubiquitin relative transcript level shows no difference(P>0.05). 6. Real Time-PCR showed that compared with the control group, mice’s hippocampus in model group’s ubiquitin m RNA expression increased. Compared with the model group, each Other groups’ expression presents decreased.Conclusion1. Kidney-essence weak and phlegm stasis is the basic etiological factor for AD, and Bushen-Huatan-Kaiqiao therapy is operative on treating AD. 2. Bushen-Huatan-Kaiqiao therapy can improve APP/PS1 double transgenic mice’s learning and memory ability. 3. Bushen-Huatan-Kaiqiao therapy can effectively improve animal hippocampal ubiquitin-proteasome pathway function.