The Mechanism Study And Biomarker Screening Of Vireous For Rhegmatogenous Retinal Detachment Associated With Choroidal Detachment

Purpose Our aim was to identify obvious different metabolites, proteins and related pathways to elucidate the etiology of rhegmatogenous retinal detachment associated with choroidal detachment (RRDCD) so as to provide direction toward diagnosis and treatment of RRDCD.Methods We used a liquid chromatography-quadrupole-time-of-flight/mass spectrometer (LC-Q-TOF/MS) to obtain the metabolome from the vitreous tissue of patients with RRD and RRDCD. The metabolomes from 29 samples(14 from RRD patients and 15 from RRDCD patients) were analyzed bySIMCA-P. A one-way analysis of variance with a Bonferroni correction was used to test significance. The Biofluid Metabolites Database and Human Metabolome Database were used to identify ions.16 vitreous samples were analysised by proteomic based on iTRAQ analysis. Proteins were analyzed by capillary high performance liquid chromatography identification and electrospray ion trap mass spectrometry after FASP digestion and iTRAQ labeling. Bioinformatics analysis were performed using Maxquant software, GO software and KEGG pathways database. The screening criteria was Ratio>+/-1.2 and P value<0.05.Result The PLS-DA identified 265 (variable importance in the project>1) ions whose levels were significantly different in vitreous from patients with RRD and RRDCD. Among the 265ions,24 (23 observed in the positive mode and 1 observed in the negative mode) were identified by searching MS and MS/MS fragments in the Biofluid Metabolites Database and Human Metabolome Database. Metabolites found were associated with pathways related to proliferation, inflammatory reactions, and hemodynamic changes. In proteomics, a total of 2510 peptides and 750 proteins were identified. There were 103 differentially expressed proteins(Ratio>+/-1.2 and P <0.05.), among which 49 proteins were up regulated and 54 proteins were down regulated. Most of the differentially expressed proteins were located in extracellular space. KEGG pathway analysis suggested that proteins related to complement and coagulation cascades were significantly enriched.Conclusions Metabolites and proteomes have been identified that were present in vitreous at significantly different levels between RRDCD and RRD. These metabolites and proteomes are likely to be involved in the pathology of each disease and may potentially be used to diagnose and treat RRDCD and RRD.