Research On The Relationship Between Emotional Factors And Endothelial Diastolic Dysfunction Of The Hypertension

ObjectivesBased on a causal relationship between psychological factors and pathogenesis of hypertension-.endothelial diastolic dysfunction,,the study aim to explore the relationship between psychological factors and endothelial diastolic function of hypertension by observing the effect of psychological factors on spontaneously hypertensive rats.MethodsTo observe influence of the emotions (fear Chi, anger Chi) for rats neuroendocrine and vascular endothelial function.1. Experiment one:the emotions stimulation (anger Chi) influence vascular endothelial function of the spontaneously hypertensive rat. We select 5 week-old male spontaneously hypertensive rats (SHR) 20 and 10 WKY rats as experimental subjects. 20 SHR rats were randomly divided into 2 groups:SHR control group, SHR anger Chi group (n= 10).10 WKY rats were served as normal controls.10 Wistar rats were an invasion group, used to irritability the SHR anger Chi group induced anger stimulation. The stimulus anger Chi the SHR anger Chi group was given the experimental factors, and monitor their behavior. SHR control group and normal control group are given normal feeding, do not give any incentives,11 days time. After the end of the intervention period, measure plasma nitric oxide (NO), plasma endothelin prime (ET) Last vasopressin Ⅱ (UT Ⅱ), thromboxane B2 (TXB2), prostaglandin (6-keto-PGF1a).2. Experiment 2:the emotions stimulation (fear Chi) influence vascular endothelial function of the spontaneously hypertensive rats. Select 20 8-week-old male spontaneously hypertensive rats (SHR) and the 10 WKY rats as experimental subjects.20 SHR rats were randomly divided into 2 groups:SHR control group, SHR fear Chi group, n= 10. Separate WKY rats 10 as the normal control group. Set and WKY rats in Group A for the normal control group, group B for SHR control group, C group is the SHR fear Chi group. Normal feeding group A and group B, do not give any incentive for 11 days; fear Chi C group was given the experimental stimuli, and monitor their behaviors. After the end of the intervention, measure plasma nitric oxide (NO), plasma endothelin prime (ET) Last vasopressin Ⅱ (UT Ⅱ), thromboxane B2 (TXB2), prostaglandin (6-keto-PGF1a). A ③ experiment 3:the emotions stimulation (fear Chi) influence vascular endothelial function of the spontaneously hypertensive rats. Use the same methods with experiment 2. after the end of the intervention period, determination of serum soluble intercellular adhesion molecule 1 (sICAM-1) and vascular cell adhesion molecule 1 (of VCAM-1) of the expression. Scanning electron microscopy the morphological changes of the thoracic aorta endothelial cells and photographed.The scanning electron microscope uses morphological description. The results establish a database of all research indicators Using SPSS 16.0 statistical software to analysis two independent samples mean comparison methods.Results1. Experiment 1:1) The rat behavior change:the start of the experiment is the adaptation period for both groups, the model group come to near the intruders several times, tentatively opponents. After the attack began, intermittently, duration ranging experiments require 20 minutes were recorded. The model rats appear biting action, after the top, climbing pressure the intruders. The intruders presents sluggish, dormant, supine posture.2)SHR anger Chi group compared with the control group, plasma nitric oxide (NO), plasma endothelin prime (ET) Last vasopressin Ⅱ (UT Ⅱ), thromboxane B2 (TXB2), prostaglandin (6-keto-PGF1a) changes were significant (P <0.05).3) SHR anger Chi group compared with SHR control group, plasma nitric oxide (NO), plasma endothelin prime (ET) Last vasopressin Ⅱ (UT Ⅱ), thromboxane B2 (TXB2), prostaglandin (6-keto -PGF1a) changes were significant (P<0.05). 4)compared to the normal control group and the SHR group, plasma nitric oxide (NO), plasma endothelin prime (ET) Last vasopressin Ⅱ (UT Ⅱ), thromboxane B2 (TXB2), prostaglandin (6-keto-PGF1a) changes were significant (P<0.05).2. Experiment Ⅱ:1) The rat behavior changes:C rats after electrical stimulation come up slow moving, adherent after huddled in the corner significantly. Still showed sluggishness, curled up, crouching freeze posture during just breathing and slight rocking motion accompanied arrectores, minor muscle tremor, stool incontinence after giving a sound stimulus without electrical stimulation. Without electrical stimulation in rats group A and group B, two groups of rats’frozen state was no significant difference (P> 0.05), and group C rats before and after electrical stimulation freeze on state time was significantly longer (P<0.01).2) A group and B group compared plasma nitric oxide (NO), plasma endothelin prime (ET),Last vasopressin II (UT II), thromboxane B2 (TXB2), prostate hormone (6-keto-PGF1a),there were significant changes (P<0.05).3) B group and C group compared plasma nitric oxide (NO), plasma endothelin prime (ET) Last vasopressin II (UT II), thromboxane B2 (TXB2), prostate hormone (6-keto-PGFla), there were significant changes (P<0.05). ③ experiment 3:1) The rat behavior changes:the same as the second experiment.2) C group compared with group A, vascular endothelial cell adhesion molecule-1 (VCAM-1), soluble intercellular adhesion molecule-1 (sICAM-1) no significant difference (P> 0.05).3) B group compared with group A, vascular endothelial cell adhesion molecule-1 (VCAM-1), soluble intercellular adhesion molecule-1 (sICAM-1) showed a significant change (P<0.05).4) scanning electron microscopy results:A group of thoracic aortic wall endothelial cells (EC) monolayer flat-shaped, fusiform banded distribution, uniform in size, shape regular, long axis take the shape and direction of blood flow, the cell membrane is more complete, The surface is relatively smooth, the nucleus area slightly elevated, neatly arranged close, mosaic arrangement of cells wide narrow portion adjacent cells, small cell gap junction shorter crest. Changes in endothelial cell morphology and structure of the group B thoracic aortic wall in varying degrees, the light, the endothelial cells basically continued to show fusiform, arranged in poor cell membrane was strip avulsion; severe, cell shrinkage smaller, irregular shaped membrane damage, or even fall off, and can see the exposed area of the sub endothelial layer, uneven cell size, sediment cells arranged in irregular, no significant directional significantly larger than normal clearance. Group C thoracic aortic wall endothelial cells arranged in a pell-mell, large and small, throughout the cells lose the original surface morphology, cell damage is serious, shedding more thoroughly, more serious damage area, the large loss of endothelial cells within cortical surface sediment more thickness ranging staggered into a network of fibrous material wrapped.ConclusionEmotional (anger Chi, fear Chi) factors can affect the blood vessels of the neuroendocrine system, causing vascular endothelial active substance disorders, leading to the destruction of vascular structures affect vascular endothelial function.