Site-specific Proteolysis Of PKGI In Non-small Cell Lung Cancer By Proprotein Convertase

Objective:To investigate the expression of PC protein and PKG-1protein in non-small cell lung cancer tissues and its relationship with clinical pathological characteristics.Methods:Immunohistochemical used to test in178cases of tumor tissue in non-small cell lung cancer for the expression of PC family protein and the PKG-1protein expression level; relationship between expression levels of each factor was analysised with gender, age, pathological type, clinical stage, lymph node metastasis, distant metastasis and other clinical parameters.Results:The positive expression rate of PC-7and furin respectively in non-small cell lung cancer were44.4%and51.7%, which were significantly higher than those in nornal tissues (P<0.05); the expression of PC-5in non-small cell lung cancer (19.1%) was lower than that of adjacent tissues (20.6%)(P>0.05); PKG-1in non-small cell lung cancer and the adjacent tissues were6.2%and57.8%(P<0.05). PC-7and furin expression in non-small cell lung cancer and clinical staging, histological differentiation and lymph node metastasis (P<0.05), positive correlation with sex and age (P>0.05); the expression of PC-5in squamous cell carcinoma rate (22.2%) was significantly lower than that of adenocarcinoma (75.6%); and the expression rate of lymph node metastasis and clinical stage (P<0.05), negative correlation with sex and age (P>0.05). PKG-1and other non-small cell lung cancer clinical pathological parameters are independent (P>0.05).Conclusion:Promote the occurrence and development of PC-7and furin may be in non-small cell lung cancer, the development of PKG-1in non-small cell lung cancer WERE independent. Objective:Overexpression of pro-protein convertase and inhibits its activity, detect the expression of cGMP dependent protein kinase, and to investigate the molecular mechanism of cGMP dependent protein kinase selectively cut.Methods:Construction of cGMP dependent protein kinase expression plasmid pCDNA3-PKG-1, inhibition of precursor protease activity by hexa-D-arginine, dec-RVKR-cmk and alpha1-PDX; overexpression of pro-protein convertase expression plasmid; immune blot was used to test the inhibition and overexpression of a pro-protein convertase conditions, changes of shear horizontal cGMP dependent protein kinase protein molecules; construction of different cGMP a little bit mutation dependent protein kinase isomers, protein targets detection precursor protein of cGMP dependent protein kinase shear. Inhibition of precursor protease activity detected by immunofluorescence after inhibition of cGMP dependent protein kinase nuclear translocation.Results:Western-bloting showed overexpression of intracellular pro-protein convertase family member furin, PC7can promote intracellular cGMP dependent protein kinase protein molecular shear, and through dec-RVKR-cmk and alpha1-PDX can inhibit the intracellular cGMP dependent protein kinase shear; through the PKG isomer of different functions. Can be found in protein kinase a precursor protease cleavage site of cGMP dependent on161amino acid at. Inhibition of the proteasome before Mars can inhibit cGMP dependent protein kinase from the cytosol into the nucleus in translocation.Conclusion:The pro-protein convertase to shearing of cGMP dependent protein kinase molecule, the splice site for the161amino acid molecules, the shear action of protein kinase molecular precursors of cGMP dependent protease is the necessary process of nuclear translocation.