Study On The Biological Activities Of Abacopteris Penangiana

Abacopteris penangiana (Hook.) Ching (Thelypteridaceae) is an ethnomedicinal herbaceous species. It is a traditional Chinese medicinal plant of Tujia and Miao. In order to reveal the biological activities of Abacopteris penangiana, the paper launched the following three research areas:1. Study on the pharmacological activities of Abacopteris penangiana;2. Quality control of total flavonoids from Abacopteris penangiana;3. Study on the protective effects of Abacopteris penangiana on prostate diseases and its mechanisms.1. we studied the pharmacological activities of Abacopteris penangiana. There are three sections in this part:(1) The purpose of this study was to evaluate anti-inflammatory acticity of Abacopteris penangiana. In vitro, LPS induced mouse microphage cell line RAW264.7was used to evaluate anti-inflammatory acticity of Abacopteris penangiana. In vivo, cotton pellets-induced granuloma model and xylene-induced ear odema model were used to assess anti-inflammatory acticity of Abacopteris penangiana. The whole results showed that Abacopteris penangiana had strong anti-inflammatory acticity. Furthermore, the anti-inflammatory activity of total flavonoids (TFA) from Abacopteris penangiana was better than total extract (AP).(2) The purpose of this study was to evaluate analgesic activity of Abacopteris penangiana. Hot plate test and acetic acid-induced abdominal writhing test was used to evaluate analgesic activity of Abacopteris penangiana. The whole results showed that Abacopteris penangiana had strong analgesic activity. Furthermore, the analgesic activity of TFA was better than AP.(3) The purpose of this study was to evaluate antiproliferative activity of Abacopteris penangiana. Methylene blue method (MTT) was used to evaluate antiproliferative activity of Abacopteris penangiana on cell lines of PC-3, MCF-7, HepG2and SH-SY5Y. The whole results showed that Abacopteris penangiana had antiproliferative activity. Furthermore, the antiproliferative activity of TFA was better than AP.2. we investigated the quality control of TFA from Abacopteris penangiana. There are two sections in this part:(1) we established a UV spectrophotometry to determine the content of total flavonoid glycosides in TFA. The results showed that Triphyllin A had the maximum absorption at wavelength of276nm. In0.0125-0.4mg/ml, Triphyllin A had a good linear relationship (R=0.9998). Moreover, the precision, stability, repeatability and recoveries met the requirement of quantitative analysis. We use the method above to determine the content of total flavonoid glycosides in TFA. The results showed that the concent of total flavonoid glycosides in TFA was69.4%.(2) we established a HPLC method to determine the content of major compounds of TFA. The results showed that Triphyllin A had a good linear relationship (R=0.9998) in0.0125-0.4mg/ml. Eruberin B had a good linear relationship (R=0.9994) in0.084-2.70mg/ml. Abacopterin I had a good linear relationship (R=0.9998) in0.05-1.60mg/ml. Moreover, the precision, stability, repeatability and recoveries met the requirement of quantitative analysis. We use the method above to determine the content of major compounds in TFA. The content of Triphyllin A, Eruberin B and Abacopterin I in TFA was118.6mg/g,96.7mg/gå'Œ53.2mg/g.3. we studied the protective effects of Abacopteris penangiana on prostate diseases and its mechanisms. There are three sections in this part:(1) The purpose of this study was to evaluate the therapeutic effects of AHT and TFA against chronic bacterial (CBP) and non-bacterial (CNP) prostatitis and explored their possible mechanisms on CNP. The whole results showed that AHT and TFA both had strong effect on CBP and CNP. The mechanisms of AHT and TFA on CNP can be described in three ways:a, AHT and TFA could modulate the activities of antioxidant enzyme. b, AHT and TFA could adjust stasis and congestion mitigation, c, AHT and TFA participated in NF-κB signaling pathway, reduced the expression of NF-κB and subsequently decreased the levels of inflammatory cytokines and pro-fibrotic factors, further reduced inflammation and fibrotic.(2) The purpose of this study was to investigate the effects of AHT and TFA on benign prostatic hyperplasia (BPH) in rats. BPH was induced in rats by subcutaneous injection of testosterone after castration. The results showed that AHT and TFA could dose dependant inhibition of BPH. The preventive effect is likely due to reducing testosterone and growth factors levels, regulating inflammatory responses, reducing oxidative stress and anti-proliferative. In addition, AHT and TFA can guard against the PI3K/Akt and NF-κB pathways in BPH.(3) The purpose of this study was to evaluate the anti-proliferative effect of flavonoid compounds from Abacopteris penangiana on PC-3cells. The MTT assay results showed that some of flavonoid compounds from Abacopteris penangiana could inhibit the viability of PC-3cells in a dose-dependent manner. Then we evaluated the relationship between the effect of monomer compounds against the proliferative activity of PC-3cells and its structure. The results showed that C-4and C-7hydroxyl has the strongest influence on the compound activity of anti-PC-3cells, followed by methyl impact in C-6and hydroxyl in C-5has no effect on the effect of compounds to against PC-3cells. Abacopterin C (AC, IC50was4.62±0.43μM) has the best effect on anti-proliferation of PC-3cells. a, Cell apoptosis was analyzed with Hoechst33258. In addition, expressions of Bcl-2and Bax were detected by western blot method. The results showed that AC increased PC-3cell apoptosis. AC reduced the expression of Bcl-2. Simultaneously, AC increased the expression of Bax. b, western blot method was used to investigate whether AC inhibits inflammatory pathway in PC3cells. The results showed that AC significantly inhibited the production of TGF-01, VEGF, TNF-α and IL-6in PC-3cells.