Study On The Active Constituents Of Anti-prostate Disease And Whitening Of Buckwheat Bee Pollen

Buckwheat bee pollen is the irregular elliptical pollen regiment collected by bees from buckwheat and mixed with nectar and bees’ secretion during the collecting process,which rich in virious nutrients needed by the human body.Buckwheat bee pollen has the efficiency of whitening,anti-inflammatory,anti-oxidation,prevention and treatment of prostate disease and so on,and it was widely used in the field of food and health products and so forth.However,the research on the material basis of various effects of buckwheat bee pollen is not perfect at present,therefore in this paper we carried out studies as follows:1.The anti-CNP(Chromc Nonbacterial Prostatitis)activity of buckwheat bee pollen.Buckwheat bee pollen was crushed,sieved,extracted and concentrated,then we obtain alcohol extract of buckwheat bee pollen.The alcohol extract of buckwheat bee pollen was applied to CNP model rats,then the results showed that it could significantly reduce the wet weight of prostate,the prostate index and the concentration of IL-8(Interleukin-8)in serum and prostate tissue homogenate of CNP model rats(P<0.01 or P<0.05),that is,the ethanol extract of buckwheat bee pollen has significant anti-CNP activity.We got five rough components separated from the ethanol extract of buckwheat bee pollen by AB-8 macroporous resin column: component A、B、C、D、E.Study on anti-CNP activity of component A、B、C、D、E showed that component C had the effect of reducing the the wet weight of prostate,prostate index,levels of IL-8 and IL-2(Interleukin-2)in serum and prostate tissue homogenate and white blood cell count of CNP model rats(P<0.01 or P<0.05).And component B also had the effect of reducing the the wet weight,prostate index,levels of IL-8 in prostate tissue homogenate and white blood cell count of CNP model rats(P<0.05).But in contrast,the overall antiinflammatory effect of component C was more pronounced than component B.Component C was further separated by MCI(Middle Chromatogram Isolated)and ODS(Octadecylsilyl)columns to give compound I、II and III.The structures were identified by NMR(Nuclear Magnetic Resonance)technique,and the compoundsⅠ 、 Ⅱ and Ⅲ were obtained as follows: 1,2,3,4,6-pen-ta-O-galloyl glucose 、 2-O-digalloyl-1,3,4,6-tetra-O-galloyl glucose、glucose that connected nine galloyl.2.The anti-BPH(Benign Prostatic Hyperplasia)activity of buckwheat bee pollen.The alcohol extract of buckwheat bee pollen was applied to BPH model mice,then the results showed that it was not significant but had a tendency to resist BPH,may be due to its complex composition,which is not much content of active ingredients and thus difficult to achieve an effective therapeutic concentration.Further studies have shown that the anti-BPH activity of each component was still not significant,but component D had some anti-BPH activity trend.Component D was further separated by MCI and ODS columns to give compound IV and compound V.The compound IV and the compound V were identified by NMR,and compound IV were obtained as: Octadecene-9Z,12 Z,15Z-trienoyl-N-ethyl glyceryl ether.The preliminary determination of compound V is monoglyceride,and its specific structure needs further study.3.The whitening activity of buckwheat bee pollen.The antioxidant activities of component A、B、C、D and E were detected by D-gal(D-galactose)induced subacute aging model mice,and the results showed that component B and component C had elevated serum SOD(Superoxide)Dismutase activity and reduced MDA(Malondialdehyde)content in liver(P<0.01 or P<0.05)in aging mice which indicating that component B and component C have significant antioxidant activity.The inhibition of tyrosinase activity by different components of buckwheat bee pollen extract showed that component C had the most significant inhibition of tyrosinase activity,component B followed,and within a certain range,their inhibition of tyrosinase was positively correlated with the concentration.Then we identified that component B was flavonoids.The three compounds isolated from the component C were further tested for inhibition of tyrosinase activity,and the results showed that,when the concentration of compound I、II、III were 1mg/m L,the inhibition rates of tyrosinase were:60.77%,78.25%,53.64%.