Study On Regulatory Effect Of MicroRNA-135a On The Th1/Th2in A Murine Model Of Allergic Rhinitis

Objective To Study on immune regulatory effect of microRNA-135a on the Thl/Th2imbalance in a murine model of allergic rhinitis(AR).Methods AR was induced in mice by ovalbumin (OVA) peritoneal injection and nasal drop in mimic, mimic control and AR groups, while saline was used in control group. Mimic and mimic control groups were treated with microRNA-135a mimic and mimic control respectively. Serum OVA-sIgE concentration was detected with ELISA method. In nasal mucosa of mice, relative mRNA and protein expressions of transcription factor (T-box expressed in T cells, T-bet&GAT A binding protein3, GATA-3) and cytokine (interferon-gamma, IFN-y&interleukin-4, IL-4) were detected with SYBR Green qPT-PCR and western-blot method. Expression of microRNA-135a in nasal mucosa of mice was detected with Bulge-LoopTM qRT-PCR method. Target validation between microRNA-135a and GATA-3was also performed in vitro.Results When serum OVA-sIgE concentration of AR group mice was higher, in the nasal mucosa, in comparison to control group, expression of microRNA-135a was lower, coincided with higher relative mRNA expressions of GATA-3, IL-4and lower expressions of T-bet, IFN-y. Compared with mimic control,293T cells co-transfected by microRNA-135a mimic and GATA-33’UTR target gene carrier decreased relative Rluc/luc fluorescence ratio; while the cell co-transfected by microRNA-135a mimic and GATA-33’UTR mutant gene carrier didn’t. After AR intervention using microRNA-135a mimic, compared with mimic control, serum OVA-sIgE concentration was lower, and in nasal mucosa expression of microRNA-135a was higher, relative mRNA and protein expressions of GATA-3and IL-4were lower, expressions of T-bet and IFN-y were higher.Conclusions MicroRNA-135a corrected the Thl/Th2imbalance in a murine model of AR probably directly through targeting of GATA-3. It provided a basis for new genetic treatments in addressing allergic diseases.