Preliminary Study Of Susceptibility MicroRNA And Its Target Gene Functional SNP In Allergic Rhinitis

Background: The incidence of allergic rhinitis(AR)is rising rapidly worldwide.Genetic susceptibility plays an important part in the individual's risk of AR.In our study,we aimed to find the regulatory mechanism in gene polymorphism related AR process.Method: MicroRNA microarray assay was performed in nasal mucosa samples from AR patients and normal subjects,and numbers of miRNAs showed altered expression compared with normal control group,moreover,some miRNAs were significantly altered with |fold change|>3 in the AR group,next we selected miRNAs that were very likely to be related to AR process based on bioinformatics data and further verified their expression level by RT-PCR technology.Novel single nucleotide polymorphism(SNP)was further identified in 3'UTR of certain target genes that had been selected and verified by bioinformatics data and dual luciferase experiments.Results: MicroRNA microarray assay and RT-PCR detection showed that hsa-miR-1244 and hsa-miR-7641 expressed lower in AR group,while hsa-miR-4651 expressed higher in AR group.Has-miR-4651 and has-miR-7641 bind with predicted target genes STAT3 and MAPK8 respectively,while has-miR-1244 could not bind with TGFB2 effectively.Sequencing 3'UTR of STAT3 and MAPK8,we found that SNP existed in 3'UTR of MAPK8 gene.Conclusion: We made the conclusion that has-miR-7641,which was related to AR,might regulate its target gene MAPK8's expression,through �SNP-miRNA-target gene aixs�,to involve in AR process.This conclusion might provide new evidence to pathogenesis and treatment research of AR.