A Research For The Mechanism Of HPAA Rhythm On The Anti-inflammatory Effect Of Moxibustion In Treating RA

Objective:To investigate anti-inflammatory effect of moxibustion treatment on the RA rats and to explore HPA axis methanism within the anti-inflammatory effect of moxibustion treatment which guided by the TCM acupuncture and moxibusti-on theory and use biological rhythm analysis as research method.Method:144health Sprague-Dawley (SD) rats were randomly divided into normal group,RA model group,moxibustion treatment group,sham group,adrenalectomy group,adrenalectomy and moxibustion treatment group(here in after referred to as"AM"),each group contains4time points (12pm group,6am group,12am gro-up,6pm group),and each time point contains6rats.To found RA animal models by Freund’s Complete Adjuvant (FCA),using LD12:12control light and dark, adrenalectomized pretreatment, do moxibustion on"Shenshu(BL23)"and"Zusanli (ST36)"poin-ts at the set time.To calculate foot swelling degree of RA rats,to determinate IL-1β、IL-6、TNF-α、CORT、ACTH、CRF in plasma by ELISA,to detect hypothalamus CRF by immunohistochemistry.Result:Study on the anti-inflammatory effect of moxibustion:1. Model group were obviously increased than normal group (P<0.05) for the amount of swelling d-egree、IL-1β、IL-6、TNF-α ACTH、CRF and hypothalamus CRF,and the amo unt of CORT obviously decreased (P<0.05).2.Moxibustion group were obviously decreased than model group (P<0.05),for the amount of swelling degree、IL-1β、IL-6、TNF-α、ACTH、CRF and hypothalamus CRF,and the amount of COR-T obviously increased (P<0.05).3.AM group were obviously increased than mox ibustion group(P<0.05) for the amount of swelling degree、IL-1β、IL-6、TNF-α、ACTH、CRF and hypothalamus CRF,and the amount of CORT obviously de-creased (P＜0.05). Study on the mechanism of moxibustion in the circadian rhythm of HPAA anti-inflammatory effect:1.The model group compared with the control group,infl-ammatory cytokins in experimental RA rats maintain circadian rhythm(P<0.05), peak phase after the shift and in the beginning of dark period,there was an inc-reasing trend of amplitude, the median was significantly increased(P<0.05);the H PA axi also maintain circadian rhythm(P<0.05),peak phase before the shift,there was an increasing trend of amplitude,the median of CORT was significantly d-ecreased(P<0.05), other HPA axis indexs was significantly increased(P<0.05).2.Th-e moxibustion group compared with the model group,inflammatory cytokines in experimental RA rats maintain circadian rhythm (P＜0.05), peak phase before th-e shift, there was an decreasing trend of amplitude, the median was significan-tly decreased(P<0.05);the HPA axis also maintain circadian rhythm(P<0.05),pea-k phase after the shift,there was an decreasing trend of amplitude,the median of CORT was significantly increased(P<0.05), other HPA axis indexs was significa-ntly decreased(P<0.05).3.The AM group compared with the moxibustion group, inflammatory cytokines in experimental RA rats lose circadian rhythm (P>0.05), peak phase after or before the shift,the amplitude closed to the moxibustion gro-up, the median was significantly increased(P<0.05);some of the HPA axi indexs lose circadian rhythm, peak phase before or after the shift, there was an increa-sing or decreasing trend of amplitude, the median of CORT was significantly decreased(P<0.05), other HPA axis indexs was significantly increased(.P<0.05).Conclusion:The anti-inflammatory effect of moxibustion on experimental RA is more explicitly.Experimental RA has obviously time characteristics of pathology.Moxib-ustion has a definite role on adjusting the experimental RA pathological rhythm. HPA axis plays an important role on adjusting the rhythm of anti-inflammatory effect in moxibustion treatment on experimental RA.