The Effects Of Chinese Herbal ShenQi Decoction On Liver MTOR/Lipin/SREBP1c Pathway In Genetically Diabetic KK-Ay Mice

Genetic factor and diet intake are important for diabetes development. Approximately70%of persons with type2diabetes mellitus have a fatty liver. However, the treatment of diabetic combined with fatty liver was still inefficiency. The present study was to perform the effects of ShenQi decoction (SQ), a Chinese herbal medicine, on blood glucose, insulin resistance, lipid profile, and liver mTOR/Lipin/SREBP1c pathway in genetically diabetic KK-Ay mice. Male mice were divided into two groups to received normal diet (ND, contain CHO71%, fat4.5%, and protein20%of calories) or high fat diet (HFD, fat34%, CHO48%, and protein17%of calories) for14weeks under free access of diet. At the9th to14th week, the HFD group was then subjected into two groups and administrated with present (HFD+SQ) or absent of SQ for6weeks. The body weight, the concentration of fasting glucose, insulin, total cholesterol, triglyceride, and the homeostasis model assessment of insulin resistance (HOMA-IR) and of beta cell function (HOMA-β) were determine before and at the end of8th,11st, and14th week of the experiment. As a result at the end of8th week, the fasting blood glucose and body weight were rapidly enhanced after1and2week high fat diet, respectively. Compared to the ND, fasting blood glucose, total cholesterol, body weight, calories intake were significantly increased in HFD. The HOMA-IR of HFD was significantly higher and HOMA-(3of HFD was significantly lower than that in ND. However, food intake of HFD was less than that in ND. As a result after the SQ administration, the fasting blood glucose, total cholesterol, triglyceride, and HOMA-IR of HFD were higher than that in ND. Moreover, HOMA-3of HFD+SQ was less than that in HFD. Furthermore, SQ decreased the HFD-induced the accumulation of lipid droplets in the liver. Moreover, SQ suppressed the HFD-induced the mTOR, Lipin, and SREBP-1c mRNA expressions, which regulated the lipogenesis. Although genetic factor is important for the diabetes onset, the appropriate macronutrient composition might to facilitate the blood glucose, lipid, and body weight control. HFD increased the blood glucose, lipid levels, and the lipid accumulation in the liver which lead the fatty liver progression and diabetes onset earlier in genetically diabetic KK-Ay mice. SQ decoction treatment might inhibit and delay the HFD induced-diabetic fatty liver severity and development partially via the hepatic mTOR/Lipin/SREBP-1c pathway. We hope that the concepts acquired from the present study will potentially provide a new consideration for treating the diabetic fatty liver via Chinese herbal medicine.