Comparative Proteome Profile Of Oogonia Meiosisinitiation And Cav1Regulated Primordial Follicle Formation In Mice

The formation primary oocytes from primordial germ cells(PGCs)through theprocess of meiosis, then interaction with pre-granulosa cells to form primordialfollicle is of vital importance for subsequent follicle formation and development, aswell as oocyte maturation processes in female mammalian. Abnormal meiosis cancause aneuploidy eventually led to the early embryonic developmentalabnormalities,embryonic death and birth defects. Oogonium meiotic defects can leadto dysgenesis, our understanding of the regulation of the meiosis initiation remainsfragmentary, especially at protein level，have not form a complete regulatory network.To better understand the molecular mechanisms involved in meiosis initiation,TMT~6-MS3technologies were used to construct a comparative proteome profile offemale mouse gonads at specific time points(11.5dpc,12.5dpc and13.5dpc),spanning a critical window inoogonium meiosis initiation. A total of3666proteinswere quantified in female gonads and473different proteins were differentiallyexpressed with a minimum fold change of two.Additionally, detailed functionalanalyses of these differentially expressed proteins wereperformed via bioinformatics.Differential proteins were enriched in mitochondrion, participate in electron transportchain.43.8%different proteins have phosphorylation modification sites,35.9%different proteins have acetylation modification sites. Elucidation of how thesechanges in protein expression level coordinate PGCs meiosis initiation is intended toprovide a better understanding of these critical biological processes early in ovariandevelopment and will provide potential therapeutic molecular targets to regulateovarian function and treat ovarian disease.In the process of gonads development, oogonium meiosis initiation andprimordial follicle formation at the sametime, the pool of primordial follicle is aresource that could be utilized throughout the reproductive life or manipulated toalleviate infertilily. The expression of Caveolin1(Cav1) bacame strongly up-regulated,in the period from11.5dpc to13.5dpc. Cav1appear to be up-regulatedin15.5dpc female gonads relative to male gonads. We examined the functional ofCav1in early gonads development process using Translation-Blocking-Vivo-Morpholino interference knockdown technology, combining with gonads invitroculture platform. Reducing15.5dpc female gonads Cav1protein expressionresulted information of abnormal follicles with more than one oocytes in a follicle. Oocyte cyststhat did not completely break apart during cysts breakdown, and thecell proliferationand migration ability is reduced. In summary, our date suggest that Cav1is required forprimordial follicle assembly and initial growth which provides an importantregulatory molecules in th process of early ovarian development.By investigate therole of cav1in primordial follicle assembly and initial growthwill provides a newcertain theoretical support to provide oocyte for infertility patients.