Hematopoietic Microenvironment Analysis Of Elderly Hip Fracture Patients Improving Risk Assessment And Fracture Prevention

Introduction:Hip fracture, frequently encounters in elderly, especially among old women, is associated with a major source of subsequent increased disability, morbidity, mortality and reduction of life quality and physical function. Although it has been the target of an enormous amount of research for several decades and much attention has been paid on prevention and mechanism of this disease, the outcome still beyond our expectation. As the incidence of hip fracture is increasing all over the world and the aging society is coming, people have to find new way and breach to decrease the incidence and improve the postoperative outcome and long-term rehabilitation.Purpose:It is widely accepted that hip fracture has a relationship with aging process. Thus, it is still poorly understood the hematopoietic microenvironment change among aging process and hip fracture. Hence, our research program creatively link hematopoietic microenvironment change, hip fracture and aging process together. Analysis the change of MAPK signalling pathway during aging process and before and after hip fracture.Methods:We isolated granulocyte from peripheral venous blood of young and old donors through MACS technology. Western blot was performed to detect the protein expression difference of three branches of MAPK signalling pathway---p-JNK, p-ERKl/2, p-p38 during aging process and hip fracture. We also detected the amount of SOD-1in our granulocyte.Results:p-JNK, p-p38was up-regulated among our old healthy controls, whereas p-p44/42was up-regulated among young healthy controls. After hip fracture, p-JNK and p-p44/42were decreased and p-p38was increasing. The amount of SOD-1in granulocyte was increasing accompany the aging process. There was nearly slight change of SOD-1in old patients, to contrast, it was growing in young patient.Conclusion:Our study creatively link MAPK signalling pathway which is important for biological activities of eukaryotes, aging process and reactive change during hip fracture together. We also found that aging process partly attributed to SOD-1swallowed by granulocyte. All of these gave a new insight and theoretical basis for risk assessment and fracture prevention of elderly in hip fracture.